Immunocytochemical study of cathepsin L and rat salivary cystatin-3 in rat osteoclasts treated with E-64 in vivo
| Autor: | Ryoji Moroi, Yukio Nishimura, Toshihiro Nishiura, Kimio Abe, Yoshihiro Terada, Teruo Tanaka, Takayoshi Yamaza, Masaru Himeno |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Male
musculoskeletal diseases Cathepsin L Bone Matrix Osteoclasts Vacuole E-64 Cysteine Proteinase Inhibitors chemistry.chemical_compound Leucine Endopeptidases Extracellular Animals Rats Wistar Salivary Proteins and Peptides Microscopy Immunoelectron General Dentistry Cathepsin biology Chemistry Cell Biology General Medicine Cathepsins Cystatins Molecular biology Rats Cysteine Endopeptidases Otorhinolaryngology Biochemistry Cytoplasm biology.protein Salivary Cystatins Cystatin Lysosomes Intracellular |
| Zdroj: | Archives of Oral Biology. 42:305-315 |
| ISSN: | 0003-9969 |
| Popis: | The localization of cathepsin L and rat salivary cystatin-3 (RSC-3) in rat osteoclasts (rat femoral and alveolar bones) treated with or without E-64 (control) was examined immunocytochemically. In osteoclasts pretreated with E-64, immunoreactivity for cathepsin L was very weak extracellularly compared to that in the control osteoclasts. However, it was strong intracellularly. The localization of RSC-3 was unclear in the control osteoclasts, while in E-64 treated osteoclasts, both the clear zone and ruffled border areas showed a very strong immunoreaction. At the electron-microscopic level, in normal osteoclasts, numerous immunoreaction products for cathepsin L were found extracellularly in the bone matrix under the ruffled border, while few intracellular products were observed. In contrast, in the E-64-treated osteoclasts, only a few immunoreaction products were found extracellularly, while intracellularly cathepsin L was found in numerous endosome-lysosomal vacuoles. In the immunoreaction for RSC-3, the cytoplasm of the ruffled border was positive, and the tips of the RSC-3-positive ruffled border appeared to enter deeply into the bone matrix. Intracellularly, the granular reaction products of RSC-3 were found in the vacuoles (probably autophagolysosomes). Thus, in E-64-treated osteoclasts, inhibition of the extracellular release of cathepsin L was demonstrated. In addition, intralysosomal accumulation of RSC-3 and deep penetration of the RSC-3-positive ruffled border into the bone matrix were found. These findings suggest that RSC-3 is associated with the inhibition of cathepsin L in both the lysosomes (in the osteoclasts) and bone matrix. |
| Databáze: | OpenAIRE |
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