The Effect of Prednisolone on miR 15a and miR16-1 Expression Levels and Apoptosis in Acute Lymphoblastic Leukemia Cell Line: CCRF-CEM
| Autor: | Majid Farshdousti Hagh, Bahman Yousefi, Malihe Najafpour, Ako Azimi, Milad Zadi Heydarabad, J. Hallajzadeh, Majid Rahnama, Arash Khorrami, A. Ghahremani |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Prednisolone Antineoplastic Agents Apoptosis Biology Flow cytometry 03 medical and health sciences 0302 clinical medicine Annexin Cell Line Tumor Drug Discovery microRNA medicine Humans Childhood Acute Lymphoblastic Leukemia medicine.diagnostic_test RNA General Medicine Precursor Cell Lymphoblastic Leukemia-Lymphoma Molecular biology Up-Regulation MicroRNAs 030104 developmental biology Cell culture 030220 oncology & carcinogenesis medicine.drug |
| Zdroj: | Drug Research. 66:432-435 |
| ISSN: | 2194-9387 2194-9379 |
| Popis: | Background: Glucocorticoids are probably the most important drugs in the treatment of childhood acute lymphoblastic leukemia (ALL). Prednisolone exerts its effect by induce apoptosis in lymphoid lineage cells. Micro RNAs are 18–24 nucleotides RNA implicated in the control of essential biological functions, including apoptosis. In the following study, the effect of prednisolone on the expression of miR 15a & miR16-1 and apoptosis in the CCRF-CEM cell line is investigated. Methods: The cell line of CCRF-CEM was cultured in standard conditions. The changes in the miR 15a and miR 16-1 expression levels were determined by Real Time-PCR technique. Also, the apoptosis is evaluated by flow cytometry using Annexin V and PI staining. Results: This study revealed that, the prednisolone induced apoptosis in a time dependent manner. Prednisolone in concentration of 700 µM was significantly increased the expression of miR 16-1 and miR 15a after 24 h and 48 h treatment (p Conclusion: prednisolone-induced apoptosis might be mediated by up-regulation of these 2 miRNAs in CCRF-CEM cells. |
| Databáze: | OpenAIRE |
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