Specificity and functional activity of anti-Burkholderia pseudomallei polysaccharide antibodies
| Autor: | M B Perry, M D Smith, P J Brett, T Schollaardt, L E Bryan, May Ho, Wipada Chaowagul |
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| Jazyk: | angličtina |
| Rok vydání: | 1997 |
| Předmět: |
Lipopolysaccharides
Blood Bactericidal Activity Melioidosis Burkholderia pseudomallei medicine.drug_class Immunology Monoclonal antibody Microbiology Antigen Antibody Specificity medicine Humans Antiserum biology O Antigens Opsonin Proteins biology.organism_classification medicine.disease Antibodies Bacterial Infectious Diseases Immunoglobulin M Polyclonal antibodies biology.protein Parasitology Antibody Research Article |
| Popis: | The lipopolysaccharide (LPS) of Burkholderia pseudomallei, the causative agent of melioidosis, consists of two O-antigenic polysaccharides designated O-PS I and O-PS II. In this study, the O-PS specificity and functional activity of a protective polyclonal antiserum and an immunoglobulin M (IgM) monoclonal antibody were determined. The polyclonal antiserum recognized both O-PS I and O-PS II, while the monoclonal antibody was O-PS II specific. Both mediated phagocytic killing of B. pseudomallei by polymorphonuclear leukocytes. Patients acutely infected with B. pseudomallei also produced antibodies to the two O-PSs, but these antibodies were not produced by asymptomatic individuals from an area of endemicity who were seropositive by an indirect hemagglutination test using sonicated heat-killed whole organisms as antigen. IgM antibodies were detected only in patients with localized infection. IgG antibodies were detected in all acutely infected patients, but there was no significant difference in antibody levels among patients with localized infection, patients who survived septicemic illness, and patients who died from septicemic illness. Further analysis of the IgG response revealed production of IgG1 and IgG2 antibodies by all patient groups, while an IgG3 response was seen only in survivors of septicemic infection. IgG4 was not detectable even when a fivefold-lower serum dilution was used. Patient sera also mediated phagocytic killing by polymorphonuclear leukocytes, and the killing effect was enhanced by complement. These results suggest that antibodies to the LPS O-polysaccharides of B. pseudomallei are protective by promoting phagocytic killing. The antibodies develop during human infection and may facilitate clearance of the organisms, as seen in a diabetic rat model of B. pseudomallei infection. |
| Databáze: | OpenAIRE |
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