ASPN and GJB2 are implicated in the mechanisms of invasion of ductal breast carcinomas
| Autor: | Castellana, Bàrbara, Escuin, Daniel, Peiró, Gloria, Garcia Valdecasas, Bárbara, Vázquez, Tania, Pons, Cristina, Pérez Olabarria, Maitane, Barnadas i Molins, Agustí, Lerma Puertas, Enrique, Universitat Autònoma de Barcelona |
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| Rok vydání: | 2021 |
| Předmět: |
Pathology
medicine.medical_specialty Invasive ductal carcinoma epithelial-mesenchymal transition ASPN Biology breast cancer Breast cancer Downregulation and upregulation Gene expression invasive ductal carcinoma medicine Epithelial–mesenchymal transition skin and connective tissue diseases Microarray analysis techniques GJB2 Ductal carcinoma medicine.disease Epithelial-mesenchymal transition in situ ductal carcinoma Oncology MMP14 Immunohistochemistry In situ ductal carcinoma Research Paper |
| Zdroj: | Recercat: Dipósit de la Recerca de Catalunya Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona Journal of Cancer Recercat. Dipósit de la Recerca de Catalunya instname |
| Popis: | The mechanism of progression from ductal carcinoma in situ (DCIS) to invasive ductal carcinoma (IDC) remains largely unknown. We compared gene expression in tumors with simultaneous DCIS and IDC to decipher how diverse proteins participate in the local invasive process. Twenty frozen tumor specimens with concurrent, but separated, DCIS and IDC were microdissected and evaluated. Total RNA was extracted and microarray analysis was performed using Affymetrix GeneChip® Human Gene 1.0 ST Arrays. Microarray data were validated by quantitative real time reverse transcription-PCR (qRT-PCR) and immunohistochemistry. Controls included seven pure in situ carcinomas, eight fragments from normal breast tissue, and a series of mouse breast carcinomas (MMTV-PyMT). Fifty-six genes were differentially expressed between DCIS and IDC samples. The genes upregulated in IDC samples, and probably associated with invasion, were related to the epithelial-mesenchymal transition (ASPN, THBS2, FN1, SPARC, and COL11A1), cellular adhesion (GJB2), cell motility and progression (PLAUR, PLAU, BGN, ADAMTS16, and ENPP2), extracellular matrix degradation (MMP11, MMP13, and MMP14), and growth/proliferation (ST6GAL2). qRT-PCR confirmed the expression patterns of ASPN, GJB2, ENPP2, ST6GAL2, and TMBS10. Expression of the ASPN and GJB2 gene products was detected by immunohistochemistry in invasive carcinoma foci. The association of GJB2 protein expression with invasion was confirmed by qRT-PCR in mouse tumors (P < 0.05). Conclusions: The upregulation of ASPN and GJB2 may play important roles in local invasion of breast ductal carcinomas. |
| Databáze: | OpenAIRE |
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