Multiple indole glucosinolates and myrosinases defend Arabidopsis against Tetranychus urticae herbivory
| Autor: | Emilie Widemann, David Letwin, Repon Kumer Saha, Cristina Rioja, Mark A. Bernards, Vladimir Zhurov, Miodrag Grbic, Brendan Walshe-Roussel, Vojislava Grbic, Vicent Arbona, Kristie Bruinsma, Aurelio Gómez-Cadenas |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Indoles Glycoside Hydrolases Physiology Glucosinolates Mutant Arabidopsis chemoprotection Plant Science 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Spider mite Botany Genetics Animals Arabidopsis thaliana Herbivory Jasmonate Tetranychus urticae two-spotted spider mite Research Articles Plant Proteins Herbivore biology herbivory biology.organism_classification defenses 030104 developmental biology chemistry Plant Defense Against Herbivory Glucosinolate feeding suppressants Tetranychidae jasmonates 010606 plant biology & botany |
| Zdroj: | Plant Physiol Repositori Universitat Jaume I Universitat Jaume I |
| Popis: | Arabidopsis (Arabidopsis thaliana) defenses against herbivores are regulated by the jasmonate (JA) hormonal signaling pathway, which leads to the production of a plethora of defense compounds. Arabidopsis defense compounds include tryptophan-derived metabolites, which limit Arabidopsis infestation by the generalist herbivore two-spotted spider mite, Tetranychus urticae. However, the phytochemicals responsible for Arabidopsis protection against T. urticae are unknown. Here, we used Arabidopsis mutants disrupted in the synthesis of tryptophan-derived secondary metabolites to identify phytochemicals involved in the defense against T. urticae. We show that of the three tryptophan-dependent pathways found in Arabidopsis, the indole glucosinolate (IG) pathway is necessary and sufficient to assure tryptophan-mediated defense against T. urticae. We demonstrate that all three IGs can limit T. urticae herbivory, but that they must be processed by myrosinases to hinder T. urticae oviposition. Putative IG breakdown products were detected in mite-infested leaves, suggesting in planta processing by myrosinases. Finally, we demonstrate that besides IGs, there are additional JA-regulated defenses that control T. urticae herbivory. Together, our results reveal the complexity of Arabidopsis defenses against T. urticae that rely on multiple IGs, specific myrosinases, and additional JA-dependent defenses. |
| Databáze: | OpenAIRE |
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