Effects of in vivo culture of murine mammary adenocarcinoma cells on tumor and metastatic growth
| Autor: | I S D'Elia, Miriam J. Diament, E. Bal de Kier Joffé, J. A. Aguirre Ghiso, Slobodanka M. Klein |
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| Rok vydání: | 1997 |
| Předmět: |
Pathology
medicine.medical_specialty Lung Neoplasms Mammary gland Biology Adenocarcinoma Metastasis Mice Immune system In vivo medicine Tumor Cells Cultured Animals Mice Inbred BALB C Proteolytic enzymes Mammary Neoplasms Experimental Metalloendopeptidases General Medicine medicine.disease Urokinase-Type Plasminogen Activator Blood Cell Count medicine.anatomical_structure Phenotype Cell culture Tumor progression Diffusion Chambers Culture Neoplasm Transplantation |
| Zdroj: | Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 18(1) |
| ISSN: | 1010-4283 |
| Popis: | We studied the effect of tumoral microenvironments on metastatic phenotypes. Therefore, murine mammary adenocarcinoma cells cultured in vivo in diffusion chambers (DC) were implanted intraperitoneally in BALB/c mice. The behavior of DC-cultured cells was compared with that of cells obtained from tumors growing subcutaneously or intraperitoneally and from primary cultures in vitro of the former. DC-cultured and control cells were inoculated into normal mice to evaluate their tumorigenicity and metastasizing ability. We found that DC-cultured cells were less tumorigenic and metastatic both in spontaneous and in experimental metastasis assays. The host response to tumor progression resulted in an early leukocytosis, probably due to the overproduction of a hematopoietic factor by the tumor cells. Finally, it was found that DC-cultured cells produced lower levels of urokinase-type plasminogen activator activity, while no differences were found in the metalloproteinase production compared to cells obtained from a tumor growing subcutaneously. |
| Databáze: | OpenAIRE |
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