The sunburn response in human skin is characterized by sequential eicosanoid profiles that may mediate its early and late phases

Autor: Anna Nicolaou, Anthony J. Thody, Desmond J. Tobin, Ann K. Haylett, Mojgan Masoodi, Lesley E. Rhodes, M Brownrigg, K Gledhill
Rok vydání: 2009
Předmět:
Male
CD3 Complex
Erythema
Lipoxygenase
Sunburn
Human skin
Pharmacology
Dinoprost
Biochemistry
Research Communications
prostaglandins
hydroxyeicosatetraenoic acids
030207 dermatology & venereal diseases
0302 clinical medicine
Tandem Mass Spectrometry
Skin
0303 health sciences
integumentary system
biology
Chemistry
Hydroxyeicosatetraenoic acid
Middle Aged
3. Good health
cyclooxygenase
Neutrophil Infiltration
Female
lipids (amino acids
peptides
and proteins)
medicine.symptom
Biotechnology
Adult
Spectrometry
Mass
Electrospray Ionization
leukocytes
Ultraviolet Rays
Inflammation
Dinoprostone
Proinflammatory cytokine
03 medical and health sciences
Genetics
medicine
Humans
Alprostadil
Molecular Biology
030304 developmental biology
Eicosanoid
Cyclooxygenase 2
Immunology
biology.protein
Eicosanoids
Cyclooxygenase
Zdroj: The FASEB Journal
ISSN: 1530-6860
0892-6638
DOI: 10.1096/fj.09-136077
Popis: Sunburn is a commonly occurring acute inflammatory process, with dermal vasodilatation and leukocyte infiltration as central features. Ultraviolet (UV) B-induced hydrolysis of membrane phospholipids releases polyunsaturated fatty acids, and their subsequent metabolism by cyclooxygenases (COXs) and lipoxygenases (LOXs) may produce potent eicosanoid mediators modulating different stages of the inflammation. Our objective was to identify candidate eicosanoids formed during the sunburn reaction in relation to its clinical and histological course. We exposed skin of healthy humans (n=32) to UVB and, for 72 h, examined expression of proinflammatory and anti-inflammatory eicosanoids using LC/ESI-MS/MS, and examined immunohistochemical expression of COX-2, 12-LOX, 15-LOX, and leukocyte markers, while quantifying clinical erythema. We show that vasodilatory prostaglandins (PGs) PGE2, PGF2α, and PGE3 accompany the erythema in the first 24–48 h, associated with increased COX-2 expression at 24 h. Novel, potent leukocyte chemoattractants 11-, 12-, and 8-monohydroxy-eicosatetraenoic acid (HETE) are elevated from 4 to 72 h, in association with peak dermal neutrophil influx at 24 h, and increased dermal CD3+ lymphocytes and 12- and 15-LOX expression from 24 to 72 h. Anti-inflammatory metabolite 15-HETE shows later expression, peaking at 72 h. Sunburn is characterized by overlapping sequential profiles of increases in COX products followed by LOX products that may regulate subsequent events and ultimately its resolution.—Rhodes, L. E., Gledhill, K., Masoodi, M., Haylett, A. K., Brownrigg, M., Thody, A. J., Tobin, D. J., Nicolaou, A. The sunburn response in human skin is characterized by sequential eicosanoid profiles that may mediate its early and late phases.
Databáze: OpenAIRE
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