Epigallocatechin-3-gallate alleviates bladder overactivity in a rat model with metabolic syndrome and ovarian hormone deficiency through mitochondria apoptosis pathways
Autor: | Bin-Nan Wu, Wen-Jeng Wu, Wan-Ting Ho, Yung-Shun Juan, Shu-Mien Chuang, Kun-Ling Lin, Yung-Chin Lee, Yi-Lun Lee |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
medicine.medical_specialty Ovariectomy lcsh:Medicine Apoptosis Inflammation Primary Ovarian Insufficiency Mitochondrion Diet High-Fat medicine.disease_cause Catechin Article Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Fibrosis Internal medicine medicine Animals lcsh:Science Metabolic Syndrome Multidisciplinary Urinary bladder Tea Plant Extracts Urinary Bladder Overactive business.industry lcsh:R medicine.disease Mitochondria Rats Disease Models Animal Oxidative Stress 030104 developmental biology Endocrinology medicine.anatomical_structure Overactive bladder 030220 oncology & carcinogenesis Female lcsh:Q Diet Carbohydrate Loading medicine.symptom Metabolic syndrome business Biomarkers Oxidative stress |
Zdroj: | Scientific Reports, Vol 8, Iss 1, Pp 1-15 (2018) Scientific Reports |
ISSN: | 2045-2322 |
DOI: | 10.1038/s41598-018-23800-w |
Popis: | Metabolic syndrome (MetS) and ovarian hormone deficiency could affect bladder storage dysfunction. Epigallocatechin-3-gallate (EGCG), a polyphenolic compound in green tea, has been shown to protect against ovarian hormone deficiency induced overactive bladder (OAB). The present study investigated oxidative stress induced by MetS and bilateral ovariectomy (OVX), and elucidated the mechanism underlying the protective effect of EGCG (10 umol/kg/day) on bladder overactivity. Rats were fed with high fat high sugar (HFHS) diet to induce MetS and received ovariectomy surgery to deprive ovarian hormone. By dieting with HFHS for 6 months, rats developed MetS and OAB. MetS + OVX deteriorated bladder storage dysfunction more profound than MetS alone. MetS and MetS + OVX rats showed over-expression of inflammatory and fibrosis markers (1.7~3.8-fold of control). EGCG pretreatment alleviated storage dysfunction, and protected the bladders from MetS and OVX - induced interstitial fibrosis changes. Moreover, OVX exacerbated MetS related bladder apoptosis (2.3~4.5-fold of control; 1.8~2.6-fold of Mets group), enhances oxidative stress markers (3.6~4.3-fold of control; 1.8~2.2-fold of Mets group) and mitochondrial enzyme complexes subunits (1.8~3.7-fold of control; 1.5~3.4-fold of Mets group). EGCG pretreatment alleviated bladder apoptosis, attenuated oxidative stress, and reduced the mitochondrial and endoplasmic reticulum apoptotic signals. In conclusions, HFHS feeding and ovarian hormone deficiency enhances the generation of oxidative stress mediated through mitochondrial pathway. EGCG reduced the generation of oxidative stress and lessened bladder overactivity. |
Databáze: | OpenAIRE |
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