Replicating Arabidopsis Model Leaf Surfaces for Phyllosphere Microbiology
| Autor: | Michal Bernach, Mitja N. P. Remus-Emsermann, Volker Nock, Rebecca Soffe |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Complex topography Surface Properties Microorganism Arabidopsis lcsh:Medicine 02 engineering and technology Microbiology Article Biomaterials 03 medical and health sciences chemistry.chemical_compound Microbial ecology Arabidopsis thaliana Dimethylpolysiloxanes lcsh:Science Microscopy Multidisciplinary biology Polydimethylsiloxane Bacteria Chemistry Replica lcsh:R fungi food and beverages 021001 nanoscience & nanotechnology biology.organism_classification Plant Leaves 030104 developmental biology Waxes lcsh:Q 0210 nano-technology Phyllosphere Nutrient agar |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019) |
| DOI: | 10.1101/523985 |
| Popis: | Artificial surfaces are commonly used in place of leaves in phyllosphere microbiology to study microbial behaviour on plant leaf surfaces. These surfaces enable a reductionist approach to be undertaken, to enable individual environmental factors influencing microorganisms to be studied. Commonly used artificial surfaces include nutrient agar, isolated leaf cuticles, and reconstituted leaf waxes. Recently, replica surfaces mimicking the complex topography of leaf surfaces for phyllosphere microbiology studies are appearing in literature. Replica leaf surfaces have been produced in agar, epoxy, polystyrene, and polydimethylsiloxane (PDMS). However, none of these protocols are suitable for replicating fragile leaves such as of the model plant Arabidopsis thaliana. This is of importance, as A. thaliana is a model system for molecular plant genetics, molecular plant biology, and microbial ecology. To overcome this limitation, we introduce a versatile replication protocol for replicating fragile leaf surfaces into PDMS. Here we demonstrate the capacity of our replication process using optical microscopy, atomic force microscopy (AFM), and contact angle measurements to compare living and PDMS replica A. thaliana leaf surfaces. To highlight the use of our replica leaf surfaces for phyllosphere microbiology, we visualise bacteria on the replica leaf surfaces in comparison to living leaf surfaces. |
| Databáze: | OpenAIRE |
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