Whole genome sequencing for drug resistance determination in Mycobacterium tuberculosis
| Autor: | Thabisile Gwala, Shaheed V. Omar, Nazir Ahmed Ismail, Halima M. Said, Farzanah Ismail, Lavania Joseph, Nabila Ismail |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Whole genome sequencing Tuberculosis drug resistance Brief Report lcsh:Public aspects of medicine 030106 microbiology Clinical Biochemistry Public Health Environmental and Occupational Health whole genome sequence Illumina miseq lcsh:RA1-1270 Drug resistance Biology biology.organism_classification medicine.disease Virology Mycobacterium tuberculosis 03 medical and health sciences Medical Laboratory Technology 0302 clinical medicine tuberculosis medicine 030212 general & internal medicine |
| Zdroj: | African Journal of Laboratory Medicine, Vol 8, Iss 1, Pp e1-e5 (2019) African Journal of Laboratory Medicine African Journal of Laboratory Medicine, Volume: 8, Issue: 1, Pages: 1-5, Published: 2019 |
| ISSN: | 2225-2010 2225-2002 |
| Popis: | South Africa remains challenged with a high tuberculosis burden accompanied by an increase in drug resistant cases. We assessed the use of the Illumina MiSeq, a next-generation sequencing platform for whole genome sequencing, followed by bioinformatic analysis using a commercial software package to determine resistance to selected drugs used for Mycobacterium tuberculosis treatment in our setting. Whole genome sequencing shows potential as a diagnostic platform for the detection of drug resistance in Mycobacterium tuberculosis with the provision of information for several drugs simultaneously. |
| Databáze: | OpenAIRE |
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