The First Specific Detection of a Highly Pathogenic Avian Influenza Virus (H5N1) in Ivory Coast
| Autor: | Yao Mathurin Koffi, Astrid Tripodi, T. Danho, F. Beudje, P. De Benedictis, Emmanuel Couacy-Hymann, Giovanni Cattoli, Sanne Charles Bodjo, Djénéba Keita, Casimir K. Kouakou |
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| Přispěvatelé: | ProdInra, Migration, Inconnu |
| Rok vydání: | 2009 |
| Předmět: |
Hemagglutination
Epidemiology [SDV]Life Sciences [q-bio] Animals Wild Biology L73 - Maladies des animaux medicine.disease_cause Sensitivity and Specificity Poultry Volaille Virus Sparrowhawk Agent pathogène virulent Grippe aviaire medicine Influenza A virus Animals Surveillance épidémiologique Influenzavirus aviaire Influenza A Virus H5N1 Subtype Raptors General Veterinary General Immunology and Microbiology Reverse Transcriptase Polymerase Chain Reaction business.industry Inoculation Public Health Environmental and Occupational Health Embryonated Oiseau Poultry farming Virology Influenza A virus subtype H5N1 [SDV] Life Sciences [q-bio] Cote d'Ivoire Infectious Diseases Influenza in Birds RNA Viral business |
| Zdroj: | Zoonoses and Public Health Zoonoses and Public Health, Wiley, 2009, 56 (1), pp.10-15 |
| ISSN: | 1863-2378 1863-1959 |
| Popis: | The Virology Laboratory of the Central Laboratory of Animal Diseases in Ivory Coast at Bingerville received samples of wild and domestic avian species between February and December 2006. An RT-PCR technique was used to test for avian influenza (AI) and highly pathogenic AI subtype viruses. Among 2125 samples, 16 were type A positive; of which, 12 were later confirmed to be H5N1. Fifteen of these 16 type A positive samples were inoculated into the chorioallantoic cavity of 11-day-old embryonated hens' eggs for virus isolation. Eight produced virus with hemagglutination titres from 1/64 to 1/512. The 4/16 M-RT-PCR positive samples, which were H5N1 negative, were shown to be H7 subtype negative. The diagnostic efficiency of the laboratory for the surveillance of H5N1 in Ivory Coast was demonstrated. The positive cases of H5N1 were from a sparrowhawk (Accipter nisus); live market poultry and in free-range poultry, where the mortality rate was approximately 20% (2/10) and 96.7% (29/30) respectively. Currently, investigations into intensive poultry farms have proved negative for H5N1. No human cases have been reported this time. |
| Databáze: | OpenAIRE |
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