Vaccine against Human Hepatitis B Virus Prepared from Antigen Derived from Human Hepatoma Cells in Culture
| Autor: | Eugene B. Buynak, Arlene A. McLean, William J. McAleer, Robert E. Weibel, D. Eugene Wampler, Maurice R. Hilleman, Henry Z. Markus, William J. Miller |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
HBsAg
Carcinoma Hepatocellular Pan troglodytes General Biochemistry Genetics and Molecular Biology Cell Line Mice Immune system Antigen medicine Animals Humans Hepatitis B Vaccines Hepatitis B Antibodies Alum adjuvant Hepatitis B Surface Antigens biology business.industry Viral Vaccine Liver Neoplasms Vaccination Viral Vaccines Hepatitis B medicine.disease Virology Immunology biology.protein Antibody business |
| Zdroj: | Experimental Biology and Medicine. 175:314-319 |
| ISSN: | 1535-3699 1535-3702 |
| DOI: | 10.3181/00379727-175-41801 |
| Popis: | Vaccine against human hepatitis B was prepared using antigen derived from hepatitis B carrier hepatoma cells grown in the interstices of a Diaflo hollow filter unit. Hepatitis B surface antigen (HBsAg) produced by these cells was purified by immune affinity chromatography, digestion with DNase and pepsin, and Sephadex G-150 separation. The Formalin-treated antigen was formulated in 20-micrograms dose on alum adjuvant with thimerosal added as a preservative. This cell culture vaccine was as potent as human plasma-derived vaccine as measured in a mouse potency assay. The vaccine proved safe in tests in chimpanzees and in human subjects who were in late stages of cancer of the central nervous system and who were receiving therapy for their condition. None of five subjects who received the vaccine developed untoward clinical reactions. Two of the subjects who received all three doses of vaccine developed antibody against HBsAg. Three persons, two given only the primary doses and one who was given all three doses but was lost to follow-up, demonstrated no response. The slow and relatively low antibody responses to the vaccine were similar to those in other immunosuppressed persons who were given vaccine of human plasma origin. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|