A set of plastid microsatellite loci for the genus Dyckia (Bromeliaceae) derived from 454 pyrosequencing
| Autor: | Tina Wöhrmann, Diego Sotero de Barros Pinangé, Bruno Huettel, Kurt Weising, Florian Krapp, Ana Maria Benko-Iseppon |
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| Rok vydání: | 2012 |
| Předmět: |
Bromeliaceae
Chloroplasts DNA Plant Molecular Sequence Data Population genetics Locus (genetics) Plant Science Polymerase Chain Reaction Dyckia Genetics Genotyping Ecology Evolution Behavior and Systematics Phylogeny DNA Primers Polymorphism Genetic biology Sequence Analysis DNA biology.organism_classification Phylogeography Chloroplast DNA Microsatellite Pyrosequencing Brazil Microsatellite Repeats |
| Zdroj: | American Journal of Botany |
| ISSN: | 1537-2197 |
| Popis: | Premise of the study: Phylogeographical analyses of Dyckia (Bromeliaceae) suffer from low levels of sequence variation. Plastid microsatellite markers were developed to achieve a better-resolved genus-wide plastid genealogy of Dyckia . Methods and Results: Approximately 84% of the D. marnier-lapostollei plastome was sequenced using 454 technology. Flanking primer pairs were designed for 34 out of 36 chloroplast simple sequence repeats (cpSSRs) detected, and 12 loci were further characterized by genotyping Dyckia samples at the level of populations and species. Three, fi ve, and six cpSSRs were polymorphic among four individuals of D. limae , 12 individuals of D. dissitifl ora , and 12 of D. pernambucana , respectively, with two to three alleles per locus and species. All loci were polymorphic among 19 different Dyckia species, with three to 10 alleles per locus. Ten primer pairs cross-amplifi ed with bromeliad genera from fi ve subfamilies. Conclusions: The set of 12 cpSSR markers provides a toolbox to analyze phylogeographical patterns of Dyckia species. |
| Databáze: | OpenAIRE |
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