Obesity- and age-related alterations in FAT/CD36 translocation and lipin-1 subcellular localization in skeletal muscle of the Zucker rats
| Autor: | Katarina Krskova, Miroslava Slamkova, Snjezana Romic, Goran Koricanac, Stefan Zorad, Viktoria Lory, Lucia Balazova, Rafał Olszanecki |
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| Rok vydání: | 2017 |
| Předmět: |
CD36 Antigens
Male 0301 basic medicine Aging medicine.medical_specialty Physiology CD36 Biophysics Skeletal muscle Inflammation Type 2 diabetes 03 medical and health sciences chemistry.chemical_compound Internal medicine medicine Animals Obesity Nuclear protein Muscle Skeletal chemistry.chemical_classification Triglyceride biology FAT/CD36 Lipin-1 Nuclear Proteins Fatty acid General Medicine Cadherins medicine.disease Rats Rats Zucker Protein Transport Cytosol 030104 developmental biology Endocrinology medicine.anatomical_structure chemistry biology.protein medicine.symptom Subcellular Fractions |
| Zdroj: | General Physiology and Biophysics |
| ISSN: | 1338-4325 |
| DOI: | 10.4149/gpb_2017010 |
| Popis: | Fatty acid (FA) uptake and/or intramuscular triglyceride (TG) accumulation in skeletal muscle are increased in obesity, type 2 diabetes and aging. FA translocase (FAT/CD36) translocation, lipin-1 subcellular localization and nuclear factor kappa B (NF-kappa B) p65 protein content in quadriceps muscle of young and old obese Zuckerfa/fa rats and their lean controls were analyzed by immunoblot to define obesity- and aging-related alterations in FA uptake, their subsequent metabolic fate and potential to activate pro-inflammatory signaling. As expected, obesity increased FAT/CD36 content in plasma membrane in quadriceps muscle of fa/fa rats. Aging increased cytosolic lipin-1 content in both, obese rats and their lean controls. Also, old obese rats had decreased level of nuclear extract lipin-lcompared to that in old lean rats. Neither obesity nor age altered NF-kappa B p65 protein content in cytosol and nuclear extract of quadriceps muscle suggesting that obesity/aging-induced changes in FA handling are not accompanied by NF-kappa B-mediated inflammation. Increase in plasma membrane FAT/CD36 content in obese rats and failure in lipin-1 export to nucleus with progression of obesity, implying an increase in FA uptake and their different channeling into lipid intermediates synthesis pathway in old fa/fa rats versus FA usage in lean rats of the same age. |
| Databáze: | OpenAIRE |
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