Lateral flow immunochromatographic assay for rapid screening of faecal carriage of carbapenemase-producing Enterobacteriaceae
| Autor: | Angélique Depouhon, Alexia Verroken, Charlotte Fauconnier, Hector Rodriguez-Villalobos, Ahalieyah Anantharajah, Magali Dodémont |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Carbapenemase-Producing Enterobacteriaceae Cost effectiveness 030106 microbiology Context (language use) Carbapenem-resistant enterobacteriaceae Sensitivity and Specificity Meropenem Chromatography Affinity beta-Lactamases Microbiology Feces 03 medical and health sciences 0302 clinical medicine Bacterial Proteins Belgium Enterobacteriaceae Limit of Detection Humans Mass Screening Medicine Pharmacology (medical) 030212 general & internal medicine Faecal carriage Pharmacology biology business.industry Enterobacteriaceae Infections Rectum biology.organism_classification Infectious Diseases Carrier State Rectal swab Reagent Kits Diagnostic business medicine.drug |
| Zdroj: | Journal of Antimicrobial Chemotherapy. 74:357-359 |
| ISSN: | 1460-2091 0305-7453 |
| Popis: | Background Rapid and effective screening of carbapenemase-producing Enterobacteriaceae (CPE) appears essential for adequate patient management and rapid implementation of infection control measures. Most of these screening techniques require a minimum of 24 h of culture. Molecular assays are an exception since these can be achieved within 1 h, but are expensive and usually require specialized facilities and trained personnel. In this context, lateral immunochromatography performed directly from rectal swab samples could represent a cost-effective alternative with a reduced turnaround time. Objectives In this study, we assessed the performance of the OKN K-SeT test (Coris BioConcept, Gembloux, Belgium) for the rapid detection of OXA-48, KPC and NDM CPE directly from rectal swab samples. Methods A total of 149 residual rectal swabs, routinely screened for CPE through selective culture and confirmed by PCR, were tested with a defined protocol consisting of a 2.5 h incubation of the swab in an enrichment medium containing meropenem followed by OKN K-SeT testing after centrifugation. Results This method displayed an overall sensitivity of 96% and a specificity of 100% with a limit of detection ranging between 104 and 105 cfu/mL. Conclusions Whereas this assay appears highly specific, it displays a reduced sensitivity compared with the standard procedure encompassing a culture step. Nonetheless, this rapid method allows an accelerated identification of most CPE carriers at a lower cost and, accordingly, the implementation of early appropriate management procedures. |
| Databáze: | OpenAIRE |
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