Interleukin 3 Promotes Histamine Synthesis in Hematopoietic Progenitors by Increasing Histidine Decarboxylase mRNA Expression
| Autor: | L.N. Gastinel, E. Schneider, Michel Dy, Atsushi Ichikawa, F. Machavoine, B. Lebel |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
medicine.medical_specialty
Population Biophysics Bone Marrow Cells In situ hybridization Histidine Decarboxylase Biology Biochemistry Mice chemistry.chemical_compound Internal medicine medicine Animals RNA Messenger Northern blot education Molecular Biology Cells Cultured In Situ Hybridization Histamine Production Interleukin 3 education.field_of_study Cell Biology Hematopoietic Stem Cells Histidine decarboxylase Molecular biology Mice Inbred C57BL medicine.anatomical_structure Endocrinology chemistry Interleukin-3 Bone marrow Histamine |
| Zdroj: | Biochemical and Biophysical Research Communications. 192:167-173 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1993.1396 |
| Popis: | Interleukin 3 (IL-3) is a potent stimulator of histamine production by cells from murine hematopoietic organs. We demonstrate herein that this phenomenon results from increased histidine decarboxylase (HDC: EC 4.1.1.22) activity in progenitor-enriched bone marrow cells (around 5% of the total bone marrow) isolated from the low density layers of a discontinuous Ficoll gradient. HDC levels are markedly enhanced after a 24 h incubation with IL-3 while a 4 h exposure results only in a slight activation. It results from increased expression of the mRNA coding for HDC, as assessed by Northern blot analysis after a 24 h incubation with IL-3. At the same time point and after a 4 h stimulation, we have evaluated the percentage of cells in this population which express HDC mRNA in response to IL-3, using in situ hybridization with the antisense riboprobe. We have thus established that enhanced HDC mRNA expression occurs in a small immature subset representing from 5 to 8% of the progenitor-enriched bone marrow cells. |
| Databáze: | OpenAIRE |
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