Novel Positively Charged Nanoparticle Labeling for In Vivo Imaging of Adipose Tissue-Derived Stem Cells
| Autor: | Ichiro Kato, Makoto Sawada, Hiroshi Yukawa, Hirofumi Noguchi, Kenji Ono, Momoko Obayashi, Yumi Hayashi, Tetsuya Ishikawa, Shingo Nakagawa, Masaki Watanabe, Yasuma Yoshizumi, Daisuke Onoshima, Yoshinobu Baba, Koichi Oishi, Shuji Hayashi, Hiroaki Saito, Noritada Kaji, Yoshitaka Miyamoto |
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| Rok vydání: | 2014 |
| Předmět: |
Pathology
medicine.medical_specialty Cell Survival Cellular differentiation Materials Science Biophysics lcsh:Medicine Adipose tissue Biology Regenerative medicine Biomaterials Mice chemistry.chemical_compound medicine Nanotechnology Animals Magnetite Nanoparticles lcsh:Science Cell Proliferation Multidisciplinary Staining and Labeling Stem Cells lcsh:R Biology and Life Sciences Cell Differentiation Magnetic Resonance Imaging Cell biology Vascular endothelial growth factor Transplantation Adipose Tissue chemistry Cell Tracking Bionanotechnology Physical Sciences Engineering and Technology Cytokines Nanoparticles Female lcsh:Q Hepatocyte growth factor Stem cell Preclinical imaging Research Article Biotechnology Stem Cell Transplantation medicine.drug |
| Zdroj: | PLoS ONE, Vol 9, Iss 11, p e110142 (2014) PLoS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0110142 |
| Popis: | Stem cell transplantation has been expected to have various applications for regenerative medicine. However, in order to detect and trace the transplanted stem cells in the body, non-invasive and widely clinically available cell imaging technologies are required. In this paper, we focused on magnetic resonance (MR) imaging technology, and investigated whether the trimethylamino dextran-coated magnetic iron oxide nanoparticle -03 (TMADM-03), which was newly developed by our group, could be used for labeling adipose tissue-derived stem cells (ASCs) as a contrast agent. No cytotoxicity was observed in ASCs transduced with less than 100 µg-Fe/mL of TMADM-03 after a one hour transduction time. The transduction efficiency of TMADM-03 into ASCs was about four-fold more efficient than that of the alkali-treated dextran-coated magnetic iron oxide nanoparticle (ATDM), which is a major component of commercially available contrast agents such as ferucarbotran (Resovist), and the level of labeling was maintained for at least two weeks. In addition, the differentiation ability of ASCs labeled with TMADM-03 and their ability to produce cytokines such as hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF) and prostaglandin E2 (PGE2), were confirmed to be maintained. The ASCs labeled with TMADM-03 were transplanted into the left kidney capsule of a mouse. The labeled ASCs could be imaged with good contrast using a 1T MR imaging system. These data suggest that TMADM-03 can therefore be utilized as a contrast agent for the MR imaging of stem cells. |
| Databáze: | OpenAIRE |
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