The overexpression of Rabl3 is associated with pathogenesis and clinicopathologic variables in hepatocellular carcinoma
| Autor: | Yi Jin, Xiang-qi Huang, Zhiyong Liu, Zhi-ying Feng, Dayang Hui, Dayue Tong, Yuhang Pan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Male Carcinoma Hepatocellular Biology Pathogenesis Small hairpin RNA 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation medicine Biomarkers Tumor Humans RNA Small Interfering RC254-282 Aged Neoplasm Staging Messenger RNA Cell growth Portal Vein Liver Neoplasms Neoplasms. Tumors. Oncology. Including cancer and carcinogens General Medicine Hep G2 Cells Middle Aged medicine.disease digestive system diseases Reverse transcription polymerase chain reaction Gene Expression Regulation Neoplastic 030104 developmental biology rab GTP-Binding Proteins 030220 oncology & carcinogenesis Hepatocellular carcinoma Lymphatic Metastasis Cancer research Immunohistochemistry Female |
| Zdroj: | Tumor Biology, Vol 39 (2017) |
| ISSN: | 1423-0380 |
| Popis: | Overexpression of Rabl3 is associated with some malignancies. However, their relationship with hepatocellular carcinoma remains unclear. In this study, the expression of Rabl3 in hepatocellular carcinoma cell lines, and four pairs of matched hepatocellular carcinoma tissues and their adjacent normal hepatic tissues were detected by quantitative reverse transcription polymerase chain reaction and western blot. In addition, the protein expression of Rabl3 was examined in 162 cases of hepatocellular carcinoma by immunohistochemistry. Rabl3 in hepatocellular carcinoma cell lines was elevated at both messenger RNA and protein levels, and the Rabl3 protein was significantly upregulated by upto 3.3-fold in hepatocellular carcinoma compared with the paired normal hepatic tissues. Immunohistochemical analysis revealed that overexpressions of Rabl3 were 80.2% in hepatocellular carcinoma. Rabl3 is expressed at significantly higher rates in hepatocellular carcinoma compared with adjacent normal hepatic tissue (p |
| Databáze: | OpenAIRE |
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