Catecholamine levels and gene expression of their receptors in tissues of adults with osteosarcoma
| Autor: | Eréndira Estrada-Villaseñor, Raúl Peralta, Alfonso Alfaro-Rodríguez, José Luis Cortes-Altamirano, Cindy Bandala, Eleazar Lara-Padilla, Alberto Avila-Luna, Noemí Cárdenas-Rodríguez, Modesto Gómez-López, Gabriela Ibáñez-Cervantes, Sergio Montes, Ángel Miliar-García, Saúl Gómez-Manzo |
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| Rok vydání: | 2019 |
| Předmět: |
Male
medicine.medical_specialty Physiology Bone Neoplasms Bone tissue Norepinephrine Catecholamines Receptors Catecholamine Dopamine Physiology (medical) Internal medicine medicine Humans Receptor Aged Osteosarcoma Chemistry General Medicine Middle Aged medicine.disease medicine.anatomical_structure Endocrinology Gene Expression Regulation Dopamine receptor Case-Control Studies Metabolome Catecholamine Female Serotonin medicine.drug |
| Zdroj: | Archives of Physiology and Biochemistry. 127:337-343 |
| ISSN: | 1744-4160 1381-3455 |
| DOI: | 10.1080/13813455.2019.1638942 |
| Popis: | Aim The purpose of this work was to identify and measure catecholamines, their metabolites, and the gene expression of catecholamine receptors in osteosarcoma tissue. Materials and methods The levels of 3,4-dihydroxyphenylacetic acid, norepinephrine, serotonin, and 5-hydroxyindoleacetic acid in cancer tissue and in adjacent and non-oncological bone tissue were analysed by high-performance liquid chromatography, and the gene expression of catecholamine receptors and of dopamine β-hydroxylase, monoaminoxidase, ki67, and Runx2 in the osteosarcoma tissue, tissue adjacent to the tumour, non-oncological bone, and human brain tissue was analysed by RT-PCR. Results We found significantly higher levels of 3,4-dihydroxyphenylacetic acid and norepinephrine in the cancer sample than in adjacent and non-oncological bone. We found that β-adrenergic receptors and dopaminergic receptors, dopamine β-hydroxylase, ki67, Runx2, and serotonergic receptor gene expression were significantly higher in tumour tissue than in adjacent and non-oncological bone. Conclusion Catecholamines and their receptors could be potential molecular markers for osteosarcoma progression. |
| Databáze: | OpenAIRE |
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