Intestinal phenotype in mice overexpressing a heparin-binding EGF-like growth factor transgene in enterocytes
| Autor: | Osama N. El-Assal, Hong Yi Zhang, Veela B. Mehta, Iyore A. Otabor, Yan Chen, Dana Wu, Andrei Radulescu, Chun Liang Chen, Gail E. Besner, Jiexiong Feng |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Heparin-binding EGF-like growth factor Enterocyte Transgene medicine.medical_treatment Clinical Biochemistry Apoptosis Mice Transgenic Article Mice Endocrinology Downregulation and upregulation medicine Animals Humans Transgenes Intestinal Mucosa Promoter Regions Genetic Cell Proliferation biology Growth factor Microfilament Proteins Cell Biology Hyperplasia medicine.disease Molecular biology Up-Regulation Intestines Enterocytes Phenotype medicine.anatomical_structure biology.protein Intercellular Signaling Peptides and Proteins Villin hormones hormone substitutes and hormone antagonists Heparin-binding EGF-like Growth Factor |
| Zdroj: | Growth Factors. 28:82-97 |
| ISSN: | 1029-2292 0897-7194 |
| Popis: | Heparin-binding EGF-like growth factor (HB-EGF) protects the intestine from damage in animals. Future clinical trials of HB-EGF may involve administration of repeated doses of HB-EGF. Since HB-EGF activates EGF receptors which have been implicated in tumor development, we examined the effects of HB-EGF overexpression in the intestine.We generated transgenic (TG) mice in which the human HB-EGF gene is driven by the villin promoter to overexpress HB-EGF along the crypt-villous axis from the duodenum to the colon.HB-EGF TG mice have increased enterocyte proliferation balanced by increased enterocyte apoptosis. Despite prolonged overexpression of HB-EGF, no evidence of intestinal hyperplasia or tumor formation occurs. Although HB-EGF TG mice have no significant phenotypic alterations under basal conditions, they have increased resistance to intestinal injury.Prolonged intestinal HB-EGF overexpression results in no significant phenotypic alterations under basal conditions, but confers protection against intestinal injury. |
| Databáze: | OpenAIRE |
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