Albumin-lysozyme interactions: Cooperative adsorption on titanium and enzymatic activity
| Autor: | Christiane Ziegler, Christine Müller-Renno, Nils Tippkötter, T. Hering, Roland Ulber, Simone Trautmann, Fabian Kratz, Matthias Hannig, Christina Rösch, Natalia Umanskaya |
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| Rok vydání: | 2017 |
| Předmět: |
Static Electricity
02 engineering and technology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Colloid and Surface Chemistry Adsorption Animals Bicinchoninic acid assay Isoelectric Point Amylase Physical and Theoretical Chemistry Enzyme Assays Titanium Gel electrophoresis Chromatography biology Chemistry Albumin Serum Albumin Bovine 030206 dentistry Surfaces and Interfaces General Medicine 021001 nanoscience & nanotechnology Enzyme assay Kinetics Isoelectric point Amylases biology.protein Cattle Muramidase Lysozyme 0210 nano-technology Protein Binding Biotechnology |
| Zdroj: | Colloids and Surfaces B: Biointerfaces. 149:115-121 |
| ISSN: | 0927-7765 |
| DOI: | 10.1016/j.colsurfb.2016.09.048 |
| Popis: | The interplay of albumin (BSA) and lysozyme (LYZ) adsorbed simultaneously on titanium was analyzed by gel electrophoresis and BCA assay. It was found that BSA and lysozyme adsorb cooperatively. Additionally, the isoelectric point of the respective protein influences the adsorption. Also, the enzymatic activity of lysozyme and amylase (AMY) in mixtures with BSA was considered with respect to a possible influence of protein-protein interaction on enzyme activity. Indeed, an increase of lysozyme activity in the presence of BSA could be observed. In contrast, BSA does not influence the activity of amylase. |
| Databáze: | OpenAIRE |
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