A recombinant fusion protein containing the domain III of the dengue-2 envelope protein is immunogenic and protective in nonhuman primates
Autor: | Mayling Alvarez, Ana B. Pérez, Jorge Martín, Aida Zulueta, María G. Guzmán, Beatriz Sierra, Delfina Rosario, Gerardo Guillén, Rafael Martínez, Laura Lazo, R. Rodríguez, Lisset Hermida, Carlos López, Irina Prado, Lidice Bernardo |
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Rok vydání: | 2006 |
Předmět: |
Recombinant Fusion Proteins
Freund's Adjuvant Enzyme-Linked Immunosorbent Assay Viremia Neisseria meningitidis Antibodies Viral medicine.disease_cause Virus Neutralization law.invention Dengue Adjuvants Immunologic Viral Envelope Proteins Neutralization Tests law Escherichia coli medicine Animals Vaccines Synthetic General Veterinary General Immunology and Microbiology biology Immunogenicity Public Health Environmental and Occupational Health Viral Vaccines Dengue Virus medicine.disease Virology Fusion protein Macaca fascicularis Infectious Diseases Immunoglobulin G biology.protein Recombinant DNA Molecular Medicine Antibody Bacterial Outer Membrane Proteins |
Zdroj: | Vaccine. 24:3165-3171 |
ISSN: | 0264-410X |
Popis: | We have previously reported the construction and evaluation in mice of recombinant fusion proteins formed by a fragment (aa 286-426) of the dengue envelope protein and the P64k protein from Neisseria meningitidis. In this work we describe the immunization of Macaca fascicularis monkeys with two variants of these proteins [PD3 (insertion variant) and PD5 (fusion variant)] corresponding to serotype 2. Four doses of the proteins adjuvated in Freund's adjuvant were administered and the kinetics of antibody induction was monitored by ELISA and neutralization tests. Monkeys receiving PD3 or PD5 developed functional antibodies (Abs) in a dose-dependent manner. Following challenge with 5 log PFU of wild type dengue-2 virus (DEN2), animals immunized with PD5 were protected from developing viremia. These results constitute a proof-of-concept demonstrating that a fragment of the dengue envelope protein, containing the domain III and produced as a recombinant fusion protein in Escherichia coli, induces functional and protective immunity in a nonhuman primate model. |
Databáze: | OpenAIRE |
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