Variability of CP4 EPSPS expression in genetically engineered soybean (Glycine max L. Merrill)
| Autor: | Keguo Huang, Gregory E. Frierdich, Luis A. Burzio, Kevin C. Glenn, Tao Geng, Adam W. Schapaugh, Duška Stojšin, Andrew E. Deffenbaugh, Zi L. Liu, Parimala Chinnadurai, Kang Liu |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Germplasm Growing region Glycine Agrobacterium Locus (genetics) Biology 01 natural sciences Expression variation 03 medical and health sciences Genetics GE crops Gene MOE Original Paper CP4 EPSPS Herbicides Genetically engineered musculoskeletal neural and ocular physiology food and beverages Drug Tolerance Plants Genetically Modified Glyphosate-tolerance Horticulture 030104 developmental biology nervous system Animal Science and Zoology Soybeans 3-Phosphoshikimate 1-Carboxyvinyltransferase Soybean Agronomy and Crop Science 010606 plant biology & botany Biotechnology |
| Zdroj: | Transgenic Research |
| ISSN: | 1573-9368 0962-8819 |
| Popis: | The expression of the CP4 EPSPS protein in genetically engineered (GE) soybean confers tolerance to the Roundup® family of agricultural herbicides. This study evaluated the variability of CP4 EPSPS expression using an enzyme-linked immunosorbent assay in soybean tissues collected across diverse germplasm and 74 different environments in Argentina, Brazil and the USA. Evaluated material included single and combined (stacked) trait products with other GE traits in entries with cp4 epsps gene at one or two loci. The highest level of CP4 EPSPS was observed in leaf tissues, intermediate in forage and seed, and lowest in root tissues. Varieties with two loci had approximately twice the level of CP4 EPSPS expression compared to one locus entries. Variable and non-directional level of CP4 EPSPS was observed with other factors like genetic background, trait stacking, growing region or season. The maximum and average CP4 EPSPS expression levels in seed provided large margins of exposure (MOE of approximately 4000 and 11,000, respectively), mitigating concerns over exposure to this protein in food and feed from soybean varieties tolerant to Roundup® herbicides. Electronic supplementary material The online version of this article (10.1007/s11248-018-0092-z) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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