The Main Structural and Functional Characteristics of Photosystem-II-Enriched Membranes Isolated from Wild Type and cia3 Mutant Chlamydomonas reinhardtii
| Autor: | Konstantin G Tikhonov, Aleksandr A Ashikhmin, Anna K Shukshina, Alexandr V. Shitov, Vasily V. Terentyev |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Photosystem II Cytochrome b559 Chlamydomonas reinhardtii macromolecular substances Photosynthesis 01 natural sciences General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound chlorophyll cytochrome b559 lcsh:Science Carotenoid Ecology Evolution Behavior and Systematics photosystem II CAH3 carotenoids OJIP-kinetic chemistry.chemical_classification biology Chemistry Wild type Paleontology food and beverages biology.organism_classification 030104 developmental biology Membrane Space and Planetary Science Chlorophyll Biophysics lcsh:Q 010606 plant biology & botany |
| Zdroj: | Life; Volume 10; Issue 5; Pages: 63 Life, Vol 10, Iss 63, p 63 (2020) |
| ISSN: | 2075-1729 |
| DOI: | 10.3390/life10050063 |
| Popis: | Photosystem II (PSII)-enriched membranes retain the original PSII architecture in contrast to PSII cores or PSII supercomplexes, which are usually isolated from Chlamydomonas reinhardtii. Here, we present data that fully characterize the structural and functional properties of PSII complexes in isolated PSII-enriched membranes from C. reinhardtii. The preparations were isolated from wild-type (WT) and CAH3-deficient mutant cia3 as the influence of CAH3 on the PSII function was previously proposed. Based on the equal chlorophyll content, the PSII-enriched membranes from WT and cia3 have the same amount of reaction centers (RCs), cytochrome b559, subunits of the water-oxidizing complex, Mn ions, and carotenes. They differ in the ratio of other carotenoids, the parts of low/intermediate redox forms of cytochrome b559, and the composition of outer light-harvesting complexes. The preparations had 40% more chlorophyll molecules per RC compared to higher plants. Functionally, PSII-enriched membranes from WT and cia3 show the same photosynthetic activity at optimal pH 6.5. However, the preparations from cia3 contained more closed RCs even at pH 6.5 and showed more pronounced suppression of PSII photosynthetic activity at shift pH up to 7.0, established in the lumen of dark-adapted cells. Nevertheless, the PSII photosynthetic capacities remained the same. |
| Databáze: | OpenAIRE |
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