Solid State NMR Investigation of Intact Human Bone Quality: Balancing Issues and Insight into the Structure at the Organic–Mineral Interface

Autor: Deepak Vashishth, Danielle Laurencin, Christian Bonhomme, Silke Besdo, Grażyna E. Sroga, Ondřej Nikel, Anna St Lorenz
Přispěvatelé: Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), department of Biomedical Engineering (RPI - BME), Rensselaer Polytechnic Institute (RPI), Laboratoire de Chimie de la Matière Condensée de Paris (site Paris VI) (LCMCP (site Paris VI)), Université Pierre et Marie Curie - Paris 6 (UPMC)-Collège de France (CdF (institution))-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institute of Continuum Mechanics, Leibniz Universität Hannover [Hannover] (LUH), Partner University Fund (PUF), Châteaubriand Fellowship, collaboration avec RPI (Troy, NY), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Leibniz Universität Hannover=Leibniz University Hannover
Rok vydání: 2012
Předmět:
Zdroj: Journal of Physical Chemistry C
Journal of Physical Chemistry C, American Chemical Society, 2012, 116, pp.6320-6331. ⟨10.1021/jp2125312⟩
Journal of Physical Chemistry C, 2012, 116, pp.6320-6331. ⟨10.1021/jp2125312⟩
ISSN: 1932-7455
1932-7447
Popis: International audience; Age-related bone fragility fractures present a significant problem for public health. Measures of bone quality are increasingly recognized to complement the conventional bone mineral density (BMD) based assessment of fracture risk. The ability to probe and understand bone quality at the molecular level is desirable in order to unravel how the structure of the organic matrix and its association with mineral contribute to the overall mechanical properties. The 13C{31P} REDOR MAS NMR (rotational echo double resonance magic angle spinning nuclear magnetic resonance) technique is uniquely suited for the study of the structure of the organic−mineral interface in bone. For the first time, we have applied it successfully to analyze the structure of intact (non-powdered) human cortical bone samples, from young healthy and old osteoporotic donors. Loading problems associated with the rapid rotation of intact bone were solved using a finite element analysis (FEA) approach, and a method allowing osteoporotic samples to be balanced and spun reproducibly is described. REDOR NMR parameters were set to allow insight into the arrangement of the amino acids at the mineral interface to be accessed, and SVD (singular value decomposition) was applied to enhance the signal-to-noise ratio and enable a better analysis of the data. From the REDOR data, it was found that carbon atoms belonging to citrate/glucosaminoglycans (GAGs) are closest to the mineral surface regardless of age or site. In contrast, the arrangement of the collagen backbone at the interface varied with site and age. The relative proximity of two of the main amino acids in bone matrix proteins, hydroxyproline and alanine, with respect to the mineral phase was analyzed in more detail and discussed in view of glycation measurements which were carried out on the tissues. Overall, this work shows that the 13C{31P} REDOR NMR approach could be used as a complementary technique to assess a novel aspect of bone quality, the organic−mineral interface structure.
Databáze: OpenAIRE
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