Quantification of sudden light-induced polarization in bacteriorhodopsin by optical rectification
| Autor: | Géza I. Groma, Manuel Joffre, Marten H. Vos, Jean-Louis Martin, Anne Colonna |
|---|---|
| Přispěvatelé: | Laboratoire d'optique et biosciences (LOB), École polytechnique (X)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Light
Infrared Infrared Rays Population 010402 general chemistry 01 natural sciences Molecular physics 03 medical and health sciences chemistry.chemical_compound Optical rectification Optics Materials Chemistry Physical and Theoretical Chemistry education 030304 developmental biology 0303 health sciences education.field_of_study biology Chemistry business.industry Bacteriorhodopsin Retinal Polarization (waves) 0104 chemical sciences Surfaces Coatings and Films Dipole Bacteriorhodopsins Femtosecond biology.protein Retinaldehyde business |
| Zdroj: | Journal of Physical Chemistry B Journal of Physical Chemistry B, American Chemical Society, 2007, 111 (10), pp.2707-2710. ⟨10.1021/jp0673462⟩ |
| ISSN: | 1520-6106 1520-5207 |
| Popis: | Upon population of its excited state, the retinal chromophore in the membrane protein bacteriorhodopsin (bR) undergoes a sudden (less than approximately 10 fs) change in dipole moment, Deltamu, that can be visualized in a direct way by optical rectification of a broadband visible femtosecond light pulse to the infrared but has not been quantified in this way. Here we show that a transparent thick AgGaS2 crystal delivers infrared radiation with the same spectral profile as bR and is a suitable reference for quantifying conversion efficiency. Using this reference, we estimate the projection of Deltamu on the membrane normal at 11 D, corresponding to the displacement of a full charge over approximately half the length of the retinal chromophore. This result may help to evaluate models describing the interplay between the initial polarization change and the subsequent isomerization of the retinal. |
| Databáze: | OpenAIRE |
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