Protective immunity against Trichinella spiralis in mice elicited by oral vaccination with attenuated Salmonella-delivered TsSP1.2 DNA
| Autor: | Peng Jiang, Jing Cui, Shu Wei Yan, Jie Feng Li, Kai Xia Guo, Jun Jun Lei, Xiang Chao Cheng, Zhong Quan Wang, Xin Qi, Yue Han, Chuan Yu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Salmonella typhimurium
0301 basic medicine Salmonella Larval Challenge [SDV]Life Sciences [q-bio] 030231 tropical medicine Trichinella spiralis Administration Oral Biology Oral Vaccination Vaccines Attenuated medicine.disease_cause DNA vaccination Microbiology Mice 03 medical and health sciences 0302 clinical medicine Immune system Intestinal mucosa Immunity parasitic diseases Vaccines DNA medicine Animals Intestinal AW Immunity Mucosal Mice Inbred BALB C lcsh:Veterinary medicine General Veterinary Vaccination fungi Zoonosis Helminth Proteins medicine.disease biology.organism_classification 3. Good health 030104 developmental biology Muscle Larvae lcsh:SF600-1100 Female Microorganisms Genetically-Modified Serine Proteases Research Article Trichinella Infection |
| Zdroj: | Veterinary Research Veterinary Research, BioMed Central, 2018, 49 (1), pp.87. ⟨10.1186/s13567-018-0582-2⟩ Veterinary Research, Vol 49, Iss 1, Pp 1-12 (2018) |
| ISSN: | 0928-4249 1297-9716 |
| DOI: | 10.1186/s13567-018-0582-2⟩ |
| Popis: | Trichinellosis is a worldwide important food-borne zoonosis caused mainly by ingesting raw or undercooked pork infected with Trichinella spiralis larvae. The development of vaccine is needed for preventing swine from Trichinella infection to ensure pork safety. Previous studies showed that T. spiralis serine protease 1.2 (TsSP1.2) is a vaccine candidate against Trichinella infection. In this study, the complete TsSP1.2 cDNA sequences were cloned into pcDNA3.1, and the rTsSP1.2 DNA was transformed into attenuated Salmonella typhimurium strain ΔcyaSL1344. Oral vaccination of mice with Salmonella-delivered rTsSP1.2 DNA vaccine induced an obvious intestinal mucosal IgA response and a systemic Th1/Th2 immune response; the vaccinated mice showed a 33.45% reduction of intestinal adult worms and 71.84% reduction of muscle larvae after T. spiralis larval challenge. The protection might be due to the rTsSP1.2-induced production of specific anti-TsSP1.2 sIgA, IgG, IgG1/IgG2a, and secretion of IFN-γ, IL-4 and IL-10, which protected intestinal mucosa from the parasite invasion, inhibited worm development and reduced female fecundity. The results indicate that the attenuated Salmonella-delivered rTsSP1.2 DNA vaccine offers a prospective strategy for the prevention and control of animal Trichinella infection. Electronic supplementary material The online version of this article (10.1186/s13567-018-0582-2) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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