Nested polymerase chain reaction for high-sensitivity detection of enteroviral RNA in biological samples

Autor: Arturo Falaschi, Giovanni Maria Severini, Mauro Giacca, Fulvio Camerini, Luisa Mestroni
Přispěvatelé: G. M., Severini, L., Mestroni, A., Falaschi, F., Camerini, Giacca, Mauro
Jazyk: angličtina
Rok vydání: 1993
Předmět:
microbiology
DNA
Sequence Homology
medicine.disease_cause
Polymerase Chain Reaction
law.invention
law
genetics/isolation /&/ purification
Sensitivity and Specificity
Sequence Homology
Dilated
genetics
Viral
Polymerase chain reaction
Enterovirus
genetics/isolation /&/ purification
Enteroviru
Heart
genetics/isolation /&/ purification
Enterovirus B
Human
Myocarditis
Evaluation Studies as Topic
RNA
Viral
Research Article
Human
Cardiomyopathy
Dilated
Microbiology (medical)
Cardiomyopathy
classification/genetics/isolation /&/ purification
Molecular Sequence Data
Biology
classification/genetics/isolation /&/ purification
Evaluation Studies as Topic
Heart
Nucleic Acid
Species Specificity
Virology
Sensitivity and Specificity
Virus
Species Specificity
Sequence Homology
Nucleic Acid
methods/statistics /&/ numerical data
Virology
Idiopathic dilated cardiomyopathy
medicine
Humans
microbiology
Humans
Molecular Sequence Data
Myocarditi
microbiology
Polymerase Chain Reaction
Base Sequence
Nucleic Acid
Base Sequence
Cardiomyopathy
genetics
Enterovirus B
genetics/isolation /&/ purification
Enterovirus
microbiology
Humans
Molecular Sequence Data
Myocarditis
methods/statistics /&/ numerical data
RNA
microbiology
RNA
DNA
Molecular biology
Reverse transcriptase
DNA
Viral
Primer (molecular biology)
Enterovirus B
Nested polymerase chain reaction
Zdroj: CIÊNCIAVITAE
Scopus-Elsevier
Europe PubMed Central
Popis: A method based on nested polymerase chain reaction was developed for the detection of enteroviral genomes in biological samples. By taking advantage of the conserved 5' noncoding region of the enteroviral RNA, two sets of primers were utilized, enabling the detection either of a broad range of enteroviruses or of group B coxsackieviruses only. The sensitivity of the method is close to the detection of single molecules of viral RNA in as much as 1 mg of tissue sample. A preliminary study showed the usefulness of this technique for the analysis of endomyocardial biopsy samples from patients with idiopathic dilated cardiomyopathy and myocarditis.
Databáze: OpenAIRE
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