Microfluidic free-flow electrophoresis chips with an integrated fluorescent sensor layer for real time pH imaging in isoelectric focusing
| Autor: | Stefan Jezierski, Stefan Nagl, Detlev Belder |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Free-flow electrophoresis
endocrine system Microfluidics Polyethylene glycol Catalysis Fluorescence Polyethylene Glycols Electrophoresis Microchip chemistry.chemical_compound Materials Chemistry Animals Microscale chemistry Fluorescent Dyes Chromatography Chemistry Isoelectric focusing Metals and Alloys Serum Albumin Bovine General Chemistry Equipment Design Hydrogen-Ion Concentration Surfaces Coatings and Films Electronic Optical and Magnetic Materials Electrophoresis Ceramics and Composites Cattle Isoelectric Focusing Layer (electronics) Conalbumin |
| Zdroj: | Chemical communications (Cambridge, England). 49(9) |
| ISSN: | 1364-548X |
| Popis: | Functional microfluidic free-flow electrophoresis chips with integrated fluorescent pH sensors are presented. Polyethylene glycol based structures were fabricated that allowed for integration of both functions on a single microchip. Microchips were applied in free-flow isoelectric focusing of model compounds and proteins with on-line monitoring of pH during microscale electrophoretic separation. |
| Databáze: | OpenAIRE |
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