Autoreactive cytotoxic T lymphocytes in human immunodeficiency virus type 1-infected subjects
| Autor: | F di Marzo Veronese, Vincenzo Barnaba, J. Shabanowitz, C B Thompson, Claudio Maria Mastroianni, Douglas J. Loftus, E Appella, A Sette, Donald F. Hunt, Kazuyasu Sakaguchi, Simonetta Salemi, David Arnott |
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| Jazyk: | angličtina |
| Rok vydání: | 1996 |
| Předmět: |
Cytotoxicity
Immunologic Viral pathogenesis T-Lymphocytes Immunology Molecular Sequence Data HIV Infections Human leukocyte antigen Major histocompatibility complex Mass Spectrometry Major Histocompatibility Complex Viral Proteins Reference Values MHC class I HLA-A2 Antigen Immunology and Allergy Cytotoxic T cell Humans Amino Acid Sequence Chromatography High Pressure Liquid B-Lymphocytes Binding Sites biology Articles Vinculin Flow Cytometry Virology Molecular biology Histocompatibility CTL biology.protein HIV-1 Peptides T-Lymphocytes Cytotoxic |
| Zdroj: | The Journal of Experimental Medicine |
| ISSN: | 1540-9538 0022-1007 |
| Popis: | A subtractive analysis of peptides eluted from major histocompatibility complex (MHC) class I human histocompatibility leukocyte antigen (HLA)-A2.1 molecules purified from either human immunodeficiency virus type-1 (HIV-1)-infected or uninfected cells was performed using micro high-performance liquid chromatography and mass spectrometry. Three peptides unique to infected cells were identified and found to derive from a single protein, human vinculin, a structural protein not known to be involved in viral pathogenesis. Molecular and cytofluorometric analyses revealed vinculin mRNA and vinculin protein overexpression in B and T lymphocytes from HIV-1-infected individuals. Vinculin peptide-specific CTL activity was readily elicited from peripheral blood lymphocytes of the majority of HLA-A2.1+, HIV+ patients tested. Our observations suggest that atypical vinculin expression and MHC class I-mediated presentation of vinculin-derived peptides accompany HIV infection of lymphoid cells in vivo, with a resultant induction of antivinculin CTL in a significant portion of HIV+ (HLA-A2.1+) individuals. |
| Databáze: | OpenAIRE |
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