The Protective Effects of Vitamins A and E on Titanium Dioxide Nanoparticles (nTiO2)-Induced Oxidative Stress in the Spleen Tissues of Male Wistar Rats

Autor: Mozhgan Afshari-Kaveh, Alireza Nourian, Nasrin Ziamajidi, Roghayeh Abbasalipourkabir
Rok vydání: 2020
Předmět:
Vitamin
Male
medicine.medical_specialty
Antioxidant
Endocrinology
Diabetes and Metabolism
medicine.medical_treatment
Clinical Biochemistry
Spleen
010501 environmental sciences
medicine.disease_cause
01 natural sciences
Biochemistry
Antioxidants
Inorganic Chemistry
Lipid peroxidation
Superoxide dismutase
03 medical and health sciences
chemistry.chemical_compound
Internal medicine
medicine
Animals
Vitamin E
Rats
Wistar
Vitamin A
0105 earth and related environmental sciences
chemistry.chemical_classification
Titanium
0303 health sciences
Glutathione Peroxidase
biology
Superoxide Dismutase
Glutathione peroxidase
030302 biochemistry & molecular biology
Biochemistry (medical)
General Medicine
Vitamins
Rats
Oxidative Stress
Endocrinology
medicine.anatomical_structure
chemistry
biology.protein
Nanoparticles
Lipid Peroxidation
Oxidative stress
Zdroj: Biological trace element research. 199(10)
ISSN: 1559-0720
Popis: Titanium dioxide nanoparticles (nTiO2) can accumulate in different tissues and damage them with oxidative stress induction. Different components with antioxidant capacity can protect the tissues. So in this study, the protective effects of vitamin A and E on the nTiO2-induced oxidative stress in rats' spleen tissues were examined. Thirty-six male Wistar rats were randomly divided into 6 groups: Control 1 (received water), nTiO2, nTiO2 + vitamin E, nTiO2 + vitamin A, nTiO2 + vitamin A and E, and Control 2 (received olive oil). To investigate the status of oxidative stress, total antioxidant capacity (TAC), total oxidant status (TOS), and lipid peroxidation (LPO) were determined in spleen tissue as well as the activities of antioxidant enzymes, including glutathione peroxidase (GPx) and superoxide dismutase (SOD). Also, the gene expression of GPx, SOD, and nuclear factor-E2-related factor-2 (Nrf-2) were determined by qRT-PCR. To evaluate the spleen histopathological changes, H&E staining was carried out. nTiO2 significantly increased TOS and LPO levels, whereas it decreased TAC level, GPx and SOD activities, and gene expression of GPx, SOD, and Nrf-2 in spleen tissues of rats compared with controls (p < 0.05). In vitamin-treated rats, the levels of TOS and LPO significantly decreased, and the level of TAC, the activities of GPx and SOD, and the gene expression of GPx, SOD, and Nrf-2 increased compared to nTiO2 group (p < 0.05). These parameters are maintained near to normal levels. Histological findings confirmed the protective effects of these vitamins on tissue damage caused by nTiO2. Vitamin A and E can protect the spleen tissues from nTiO2-induced oxidative stress.
Databáze: OpenAIRE
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