Delineation of an epitope recognized by a chikungunya virus anti-capsid monoclonal antibody on the protease domain using an immuno-informatics approach
| Autor: | Megha Agrawal, Rekha Damle, Daya Pavitrakar, Aabha Thite, Sarah S. Cherian |
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| Rok vydání: | 2021 |
| Předmět: |
medicine.drug_class
viruses medicine.medical_treatment 030303 biophysics Biology Antibodies Viral medicine.disease_cause Monoclonal antibody Virus Epitope Domain (software engineering) Epitopes 03 medical and health sciences Structural Biology medicine Humans Chikungunya Molecular Biology 0303 health sciences Protease Antibodies Monoclonal virus diseases General Medicine biochemical phenomena metabolism and nutrition Virology Capsid Informatics Chikungunya Fever Capsid Proteins Chikungunya virus Epitope Mapping Peptide Hydrolases |
| Zdroj: | Journal of Biomolecular Structure and Dynamics. 40:5623-5633 |
| ISSN: | 1538-0254 0739-1102 |
| Popis: | The capsid-protein (CP) of chikungunya virus (CHIKV) is reported to generate a primary immune response in infected individuals during disease progression. CP-specific monoclonal antibodies (mAbs) developed in our laboratory, exhibited promising potential in diagnosing recent CHIKV infection in IgM capture ELISA. In this study we focused on the molecular and structural characterization of one such representative mAb ClVE4/D9 to delineate the epitope recognized by it using an immuno-informatics approach. The antigen-antibody interacting residues were found to lie within the dimer interface region of the CP, also predicted as a conformational epitope. This implies that the mAb could interfere during the process of nucleocapsid assembly, ultimately preventing budding and egress of the virus particle. The binding specificity of the mAb highlights the possibility of using this anti-CP antibody for therapeutic or prophylactic treatment against CHIKV.Communicated by Ramaswamy H. Sarma. |
| Databáze: | OpenAIRE |
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