Establishment and characterization of a new mantle cell lymphoma cell line, Mino
| Autor: | L. J. Medeiros, Tyler J. Curiel, Timothy J. McDonnell, R Oudat, Raymond Lai, Sean L. O'Connor, M. Keating, Richard J. Ford, M. B. Morgan |
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| Rok vydání: | 2002 |
| Předmět: |
Cancer Research
Herpesvirus 4 Human Tumor suppressor gene Blotting Western Mutation Missense Cell Cycle Proteins Lymphoma Mantle-Cell Biology medicine.disease_cause Polymerase Chain Reaction Immunophenotyping Cyclin D1 Fatal Outcome Cyclin D2 immune system diseases Antigens CD hemic and lymphatic diseases Cyclins medicine Tumor Cells Cultured Humans Point Mutation Cyclin D3 Codon neoplasms In Situ Hybridization Fluorescence Cell Size Chromosome Aberrations Retinoblastoma protein Hematology Exons Middle Aged medicine.disease Aneuploidy Genes p53 Epstein–Barr virus Neoplasm Proteins Oncology Amino Acid Substitution Karyotyping Cancer research biology.protein Mantle cell lymphoma Female CD5 |
| Zdroj: | Leukemia research. 26(9) |
| ISSN: | 0145-2126 |
| Popis: | Mantle cell lymphoma (MCL) is a distinct type of B-cell non-Hodgkin’s lymphoma characterized by cyclin D1 overexpression and the cytogenetic abnormality, the t(11;14)(q13;q32). MCL cell lines have been difficult to establish and in vitro studies of these neoplasms are scarce. We describe the establishment and characteristics of a new MCL cell line, Mino. The cells are large, growing singly and in small clumps in vitro. By flow cytometry, the immunophenotype was compatible with MCL (i.e. CD5 + CD20 + CD23 − FMC7 + ). Conventional cytogenetics showed hyperdiploidy with multiple complex karyotypic abnormalities, but no evidence of the t(11;14), proven to be present only by fluorescence in situ hybridization and polymerase chain reaction (PCR) methods. Western blots showed expression of cyclin D1 but no detectable cyclin D2 and cyclin D3; the retinoblastoma protein was predominantly phosphorylated. There was expression of tumor suppressor gene products including p53, p16 INK4a , and p21 WAF1 . Sequencing of the TP53 gene revealed a mutation (codon 147 valine→glycine ) in exon 5. Epstein Barr virus was absent. In summary, Mino is a new MCL cell line that may be useful to study the pathogenesis of MCL. |
| Databáze: | OpenAIRE |
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