Isoelectric focusing of rabbit IgM hemolysin: Heterogeneity with respect to charge and function
| Autor: | Stephen C. Elliott, Chao Y. Kuo, Louis G. Hoffmann |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
Erythrocytes
Immunology Immunoelectrophoresis Hemolysin Proteins medicine Animals Immunology and Allergy Antigens Edetic Acid Aminocaproates Sheep biology medicine.diagnostic_test Chemistry Isoelectric focusing Dextrans Hemolysin Hemagglutination Tests Molecular biology Isoelectric point Immunoglobulin M Sephadex Chromatography Gel biology.protein Rabbits Isoelectric Focusing Antibody |
| Zdroj: | Journal of Immunological Methods. 1:391-398 |
| ISSN: | 0022-1759 |
| Popis: | Isoelectric focusing can be used to purify rabbit IgM immunoglobulins. In this study, it has been applied to hemolytic IgM antibody, obtained from pooled sera of immunized rabbits by recycling chromatography on Sephadex G-200. The IgM molecules show a range of apparent isoelectric points from 4.7 to 5.6, with a peak of hemolytic activity at pI = 5.2. The hemolytic and hemagglutinating activities do not coincide; the ratio of hemolytic to hemagglutinating activities increases 3-fold from the low to the high end of the isoelectric distribution. |
| Databáze: | OpenAIRE |
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