A novel dressing for the combined delivery of platelet lysate and vancomycin hydrochloride to chronic skin ulcers: Hyaluronic acid particles in alginate matrices
| Autor: | Giuseppina Sandri, Silvia Rossi, Maria Cristina Bonferoni, Franca Ferrari, Federica Riva, Barbara Vigani, Carla Caramella, Michela Mori |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Adult
Blood Platelets Calcium alginate Alginates Vancomycin Hydrochloride Pharmaceutical Science 02 engineering and technology 030226 pharmacology & pharmacy 03 medical and health sciences chemistry.chemical_compound Drug Delivery Systems 0302 clinical medicine Glucuronic Acid Vancomycin Skin Ulcer Hyaluronic acid medicine Humans Hyaluronic Acid Cells Cultured Cell Proliferation Skin Wound Healing Chromatography Hexuronic Acids Biological activity Fibroblasts 021001 nanoscience & nanotechnology Bandages In vitro Anti-Bacterial Agents chemistry Platelet lysate 0210 nano-technology Ex vivo medicine.drug |
| Zdroj: | European Journal of Pharmaceutical Sciences. 118:87-95 |
| ISSN: | 0928-0987 |
| Popis: | The aim of the present work was to develop a medication allowing for the combined delivery of platelet lysate (PL) and an anti-infective model drug, vancomycin hydrochloride (VCM), to chronic skin ulcers. A simple method was set up for the preparation of hyaluronic acid (HA) core-shell particles, loaded with PL and coated with calcium alginate, embedded in a VCM containing alginate matrix. Two different CaCl2 concentrations were investigated to allow for HA/PL core-shell particle formation. The resulting dressings were characterized for mechanical and hydration properties and tested in vitro (on fibroblasts) and ex-vivo (on skin biopsies) for biological activity. They were found of sufficient mechanical strength to withstand packaging and handling stress and able to absorb a high amount of wound exudate and to form a protective gel on the lesion area. The CaCl2 concentration used for shell formation did not affect VCM release from the alginate matrix, but strongly modified the release of PGFAB (chosen as representative of growth factors present in PL) from HA particles. In vitro and ex vivo tests provided sufficient proof of concept of the ability of dressings to improve skin ulcers healing. |
| Databáze: | OpenAIRE |
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