Studies on the changes of uPA system in a co-culture model of bone marrow stromal cells-leukemia cells
| Autor: | Fang Jia, Shouliang Dong, Hong Guo, Li Zhao, Xuan Chen, Xiaowei Zhang, Lanxia Zhou |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Vascular Endothelial Growth Factor A Cell Communication Biochemistry chemistry.chemical_compound 0302 clinical medicine Tumor Microenvironment Child Research Articles Cancer Leukemia vascular endothelial growth factor Middle Aged Vascular endothelial growth factor medicine.anatomical_structure Matrix Metalloproteinase 9 HL60 030220 oncology & carcinogenesis Plasminogen activator inhibitor-1 Female Signal Transduction Adult Stromal cell Adolescent Biophysics HL-60 Cells Receptors Urokinase Plasminogen Activator HS-5 03 medical and health sciences Young Adult stomatognathic system Plasminogen Activator Inhibitor 1 medicine Humans Molecular Biology Tumor microenvironment Membrane Proteins Mesenchymal Stem Cells Cell Biology medicine.disease Urokinase receptor 030104 developmental biology chemistry matrix metalloproteins-9 Cancer research Bone marrow K562 K562 Cells |
| Zdroj: | Bioscience Reports |
| ISSN: | 1573-4935 |
| Popis: | The core of the tumor microenvironment in the hematological system is formed by bone marrow stromal cells (BMSCs). In the present study, we explored the interaction between the urokinase plasminogen activator (uPA) system and the leukemia bone marrow microenvironment (BMM). We established BMSCs–HL60 and HS-5–K562 co-culture models in direct contact mode to simulate the BMM in leukemia. In BMSCs-HL60 co-culture model, the expression levels of uPA, uPA receptor (uPAR), plasminogen activator inhibitor 1 (PAI-1) and vascular endothelial growth factor (VEGF) in BMSCs were higher than those in mono-cultured BMSCs. Matrix metalloproteinase (MMP)-9 (MMP-9) was up-regulated in co-cultured HL60 cells. In HS-5–K562 co-culture model, only uPA, PAI-1, and VEGF-A were up-regulated in HS-5 cells. The levels of the uPA protein in the co-culture supernatant were significantly higher than that of mono-cultured BMSCs or HS-5 cells. Our findings demonstrate that the co-culture stimulates the production of uPA, uPAR, PAI-1, MMP-9, and VEGF-A by BMSCs. It could further explain how the uPA system in leukemia cells is involved in the growth, development, and prognosis of leukemia. |
| Databáze: | OpenAIRE |
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