Identification of a variant hotspot in 'MYBPC3' and of a novel 'CSRP3' autosomal recessive alteration in a cohort of Polish patients with hypertrophic cardiomyopathy
| Autor: | Irene Bottillo, Lidia Tomkiewicz-Pajak, Paola Grammatico, Paweł Petkow-Dimitrow, Ewa Wypasek, Francesco Binni, Paweł Rubiś, Anetta Undas, Luigi Laino, Diana Giannarelli, Martina Lipari, Marek Karpiński |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Adult
Male 0301 basic medicine Adolescent DNA Mutational Analysis Cardiomyopathy Muscle Proteins Compound heterozygosity Young Adult 03 medical and health sciences Heart disorder 0302 clinical medicine Internal Medicine Humans Medicine Missense mutation Child CSRP3 Gene Aged CSRP3 human KO hypertrophic cardiomyopathy MYBPC3 founder mutation Polish population Genetics business.industry Hypertrophic cardiomyopathy High-Throughput Nucleotide Sequencing Infant Cardiomyopathy Hypertrophic LIM Domain Proteins Middle Aged medicine.disease 030104 developmental biology Child Preschool 030220 oncology & carcinogenesis Mutation Female Poland Carrier Proteins business |
| Popis: | Introduction Hypertrophic cardiomyopathy (HCM) is a heart disorder caused by autosomal dominant alterations affecting both sarcomeric genes and other nonsarcomeric loci in a minority of cases. However, in some patients, the occurrence of the causal pathogenic variant or variants in homozygosity, compound heterozygosity, or double heterozygosity has also been described. Most of the HCM pathogenic variants are missense and unique, but truncating mutations of the MYBPC3 gene have been reported as founder pathogenic variants in populations from Finland, France, Japan, Iceland, Italy, and the Netherlands. Objectives This study aimed to assess the genetic background of HCM in a cohort of Polish patients. Patients and methods Twenty‑nine Polish patients were analyzed by a next generation sequencing panel including 404 cardiovascular genes. Results Pathogenic variants were found in 41% of the patients, with ultra‑ rare MYBPC3 c.2541C>G (p.Tyr847Ter) mutation standing for a variant hotspot and correlating with a lower age at HCM diagnosis. Among the nonsarcomeric genes, the CSRP3 mutation was found in a single case carrying the novel c.364C>T (p.Arg122Ter) variant in homozygosity. With this finding, the total number of known HCM cases with human CSRP3 knockout cases has reached 3. Conclusions This report expands the mutational spectrum and the inheritance pattern of HCM. |
| Databáze: | OpenAIRE |
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