Serologic markers in early stages of African horse sickness virus infection
| Autor: | M. Diaz-Laviada, C. Vela, J I Casal, José Manuel Sánchez-Vizcaíno, Jorge L. Martínez-Torrecuadrada, Carmen Navarro Sánchez, Polly Roy |
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| Předmět: |
Microbiology (medical)
Serotype viruses African Horse Sickness Virus Blotting Western Biology Antibodies Viral Virus Serology Viral Proteins Capsid African Horse Sickness Animals Antigens Viral Orbivirus Vaccination virus diseases Viral Vaccines biochemical phenomena metabolism and nutrition biology.organism_classification Virology Immunology biology.protein African horse sickness Capsid Proteins Viral disease Antibody Research Article |
| Zdroj: | Scopus-Elsevier ResearcherID |
| Popis: | Fifteen horses were experimentally infected with African horse sickness virus (AHSV) serotype 4. To learn more about the time course of production and specificity of AHSV-specific antibodies, sera were analyzed by immunoblot analysis. Only animals that survived for more than 9 days were able to develop a humoral immune response detectable by immunoblotting. The earliest serological markers corresponded mainly to VP5, VP6, and NS2 and to a lesser extent to VP3, NS1, and NS3. Neutralizing antibodies to VP2 were not detected by immunoblotting, suggesting that they are mostly conformation dependent. VP7-specific antibodies were detected later in infection. These results make NS2 and VP6 the most attractive candidates for the rapid diagnosis of the infection. |
| Databáze: | OpenAIRE |
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