Pathogenic characteristics of a novel intranuclear coccidia in Japanese black calves and its genetic identification as Eimeria subspherica
| Autor: | Kazumi Sasai, Makoto Matsubayashi, Naoto Imai, Yoshimasa Hirashima, Ayako Okada, Shogo Tanaka, Tomo Kawakami, Tomoyuki Shibahara, Terunori Koreeda |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty 040301 veterinary sciences Cattle Diseases Eimeria 18S ribosomal RNA Microbiology 0403 veterinary science 03 medical and health sciences Feces Coccidia parasitic diseases medicine RNA Ribosomal 18S Animals Phylogeny DNA Primers General Veterinary biology Phylogenetic tree Coccidiosis 04 agricultural and veterinary sciences General Medicine Ribosomal RNA biology.organism_classification medicine.disease Cyclospora 030104 developmental biology Infectious Diseases Jejunum Parasitology Insect Science Cattle |
| Zdroj: | SC30201804040008 NARO成果DBa |
| ISSN: | 0932-0113 |
| Popis: | Bovine intranuclear coccidiosis is caused by the protozoans Eimeria alabamensis and Cyclospora spp. Here, we characterized the disease and genetically identified the causative species in Japanese black calves with chronic and refractory watery diarrhea. Histologic examinations revealed atrophy of the jejunal villi and numerous parasites in the nucleus of epithelial cells in the jejunum. Based on molecular analyses using 18S ribosomal RNA gene-specific primers that we designed, the parasites were found to be formed in the same cluster as Eimeria subspherica in the phylogenetic tree, which was separated from those of other related Eimeria spp. These results constitute the first report of E. subspherica as a cause of bovine intranuclear coccidiosis. |
| Databáze: | OpenAIRE |
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