Histological evaluation of sheep ovarian tissue after laparoscopic partial ovariectomy
| Autor: | Pedro Soares Bezerra Júnior, Filipe Luigui Soares da Costa, Janayna Barroso dos Santos, Barbara da Conceição Guilherme, Chayanne Silva Ferreira, Pedro Paulo Maia Teixeira, Maria Jeane da Silva Cavalcante, Hanna Lyce Magno de Morais, Luisa Pucci Bueno Borges, Michel Santos Cunha, Adriana Elizabeth Cordeiro Barbosa, Rodrigo dos Santos Albuquerque, Marco Augusto Machado Silva, Daniella Kaísa de Oliveira Bezerra, Renato Abrantes Oliveira, Gabriela Melo Alves dos Santos, Carmen Silvia Pantoja Pereira, Kayan Cunha Rossy, Luciana da Silva Siqueira, Carla Rozilene Guimarães Silva |
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| Rok vydání: | 2019 |
| Předmět: |
Pathology
medicine.medical_specialty Ovariectomy Healthy tissue Surgical methods Bipolar forceps 03 medical and health sciences 0302 clinical medicine Endocrinology Food Animals Tissue damage Biopsy medicine Animals Laparoscopy Sheep 030219 obstetrics & reproductive medicine medicine.diagnostic_test business.industry Ovarian tissue Ovary 0402 animal and dairy science 04 agricultural and veterinary sciences General Medicine 040201 dairy & animal science Female Animal Science and Zoology business |
| Zdroj: | Animal Reproduction Science. 204:165-170 |
| ISSN: | 0378-4320 |
| Popis: | In the present study, there was assessment of the damage to tissue caused by partial laparoscopic ovariectomy using bipolar forceps in sheep. Fragments of ovaries of six sheep were removed using bipolar forceps by making a transverse section in the middle third of the organ via three-portals that were made using laparoscopy. The fragments were subjected to standard histological examinations and the lesions attributed to the procedure were investigated using an optical microscope and Image J software. The results were assessed using an analysis of variance and the Tukey test. All the laminae had minimal tissue damage. The mean amount of highly damaged tissue was 1.8%, and of partially damaged tissue was 5.6%. The mean total area of healthy tissue in the fragments was 94.4%. The results of the study indicate this procedure can be conducted withvery little tissue damage occurring. The use of this procedure, therefore, can be incorporated in future reproductive studies without altering the functions of the in situ ovarian tissues. |
| Databáze: | OpenAIRE |
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