Induction of Microsomal Prostaglandin E2 Synthase in the Macula Densa in Children with Hypokalemic Salt-Losing Tubulopathies
| Autor: | Martin Kömhoff, Stephan C. Reinalter, Hermann Josef Gröne, Hannsjörg W. Seyberth |
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| Rok vydání: | 2004 |
| Předmět: |
Male
medicine.medical_specialty Adolescent medicine.medical_treatment Hypokalemia 6-Ketoprostaglandin F1 alpha Bartter syndrome Dinoprostone Antibody Specificity Microsomes Internal medicine Renin Renin–angiotensin system medicine Humans Prostaglandin E2 Child Prostaglandin-E Synthases Chemistry Membrane Proteins medicine.disease Immunohistochemistry Juxtaglomerular Apparatus In vitro Intramolecular Oxidoreductases Isoenzymes Kidney Tubules Endocrinology medicine.anatomical_structure Eicosanoid Cyclooxygenase 2 Prostaglandin-Endoperoxide Synthases Child Preschool Pediatrics Perinatology and Child Health Microsome Macula densa Female Kidney Diseases Salts lipids (amino acids peptides and proteins) Signal Transduction Prostaglandin E medicine.drug |
| Zdroj: | Pediatric Research. 55:261-266 |
| ISSN: | 1530-0447 0031-3998 |
| DOI: | 10.1203/01.pdr.0000101747.09626.6b |
| Popis: | In hyperprostaglandin E syndrome (HPGES) and classic Bartter syndrome (cBS), tubular salt and water losses stimulate renin secretion, which is dependent on enhanced cyclooxygenase-2 (COX-2) enzymatic activity. In contrast to other renal COX metabolites, only prostaglandin E(2) (PGE(2)) is selectively up-regulated in these patients. To determine the intrarenal source of PGE(2) synthesis, we analyzed the expression of microsomal PGE(2) synthase (mPGES; EC: 5.3.99.3), whose product PGE(2) has been shown to stimulate renin secretion in vitro. Expression of mPGES was analyzed by immunohistochemistry in eight patients with HPGES, in two patients with cBS, and in six control subjects. Expression of mPGES immunoreactive protein was observed in cells of the macula densa in five of eight HPGES patients and in one of two cBS patients. Expression of mPGES immunoreactive protein was not observed in cells associated with the macula densa in kidneys from control subjects without a history consistent with activation of the renin angiotensin system. Co-induction of COX-2 and mPGES in cells of the macula densa suggests that PGE(2) activates renin secretion in humans. |
| Databáze: | OpenAIRE |
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