Treatment of Silk Fibroin with Poly(ethylene glycol) for the Enhancement of Corneal Epithelial Cell Growth
| Autor: | Rebecca A. Dawson, Grant Edwards, Thomas A. Hogerheyde, Damien G. Harkin, Traian V. Chirila, Audra M. A. Shadforth, Shuko Suzuki |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Materials science
porosity lcsh:Biotechnology 111301 Ophthalmology Biomedical Engineering Silk fibroin Fibroin Nanotechnology macromolecular substances Article Cell therapy Cornea Biomaterials 100404 Regenerative Medicine (incl. Stem Cells and Tissue Engineering) chemistry.chemical_compound Bombyx mori lcsh:TP248.13-248.65 PEG ratio silk Corneal transplantation lcsh:R5-920 biology poly(ethylene glycol) Cell growth fungi Bombyx mori silk fibroin technology industry and agriculture cell attachment surface topography biology.organism_classification Membrane chemistry Permeability (electromagnetism) membranes corneal epithelial cells 090301 Biomaterials Genipin Biophysics permeability lcsh:Medicine (General) Ethylene glycol |
| Zdroj: | Journal of Functional Biomaterials Volume 6 Issue 2 Pages 345-366 Journal of Functional Biomaterials, Vol 6, Iss 2, Pp 345-366 (2015) |
| ISSN: | 2079-4983 |
| DOI: | 10.3390/jfb6020345 |
| Popis: | A silk protein, fibroin, was isolated from the cocoons of the domesticated silkworm (Bombyx mori) and cast into membranes to serve as freestanding templates for tissue-engineered corneal cell constructs to be used in ocular surface reconstruction. In this study, we sought to enhance the attachment and proliferation of corneal epithelial cells by increasing the permeability of the fibroin membranes and the topographic roughness of their surface. By mixing the fibroin solution with poly(ethylene glycol) (PEG) of molecular weight 300 Da, membranes were produced with increased permeability and with topographic patterns generated on their surface. In order to enhance their mechanical stability, some PEG-treated membranes were also crosslinked with genipin. The resulting membranes were thoroughly characterized and compared to the non-treated membranes. The PEG-treated membranes were similar in tensile strength to the non-treated ones, but their elastic modulus was higher and elongation lower, indicating enhanced rigidity. The crosslinking with genipin did not induce a significant improvement in mechanical properties. In cultures of a human-derived corneal epithelial cell line (HCE-T), the PEG treatment of the substratum did not improve the attachment of cells and it enhanced only slightly the cell proliferation in the longer term. Likewise, primary cultures of human limbal epithelial cells grew equally well on both non-treated and PEG-treated membranes, and the stratification of cultures was consistently improved in the presence of an underlying culture of irradiated 3T3 feeder cells, irrespectively of PEG-treatment. Nevertheless, the cultures grown on the PEG-treated membranes in the presence of feeder cells did display a higher nuclear-to-cytoplasmic ratio suggesting a more proliferative phenotype. We concluded that while the treatment with PEG had a significant effect on some structural properties of the B. mori silk fibroin (BMSF) membranes, there were minimal gains in the performance of these materials as a substratum for corneal epithelial cell growth. The reduced mechanical stability of freestanding PEG-treated membranes makes them a less viable choice than the non-treated membranes. |
| Databáze: | OpenAIRE |
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