Effect of Bisphosphonate Pretreatment on Fresh Osteochondral Allografts: Analysis of In Vitro Graft Structure and In Vivo Osseous Incorporation
| Autor: | Drew D. Moore, Mackenzie M Fleischer, Kevin C. Baker, Zachary M. Vaupel, Paul T. Fortin, Nicholas A Barreras, Michael D. Newton, Erin A. Baker |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
Cell Survival Swine medicine.medical_treatment Miniature swine Cold storage Pilot Projects In Vitro Techniques Chondrocyte Bone resorption 03 medical and health sciences Random Allocation 0302 clinical medicine Chondrocytes In vivo Osseointegration Outcome Assessment Health Care Medicine Animals Humans Transplantation Homologous Orthopedics and Sports Medicine Femur 030203 arthritis & rheumatology 030222 orthopedics Bone Transplantation Bone Density Conservation Agents Diphosphonates business.industry Bisphosphonate Allografts Transplantation medicine.anatomical_structure Surgery Female Tissue Preservation business Cadaveric spasm Biomedical engineering |
| Zdroj: | Orthopedics. 41(3) |
| ISSN: | 1938-2367 |
| Popis: | Fresh allograft transplantation of osteochondral defects restores functional articular cartilage and subchondral bone; however, rapid loss of chondrocyte viability during storage and osteoclast-mediated bone resorption at the graft–host interface after transplantation negatively impact outcomes. The authors present a pilot study evaluating the in vitro and in vivo impact of augmenting storage media with bisphosphonates. Forty cylindrical osteochondral cores were harvested from femoral condyles of human cadaveric specimens and immersed in either standard storage media or storage media supplemented with nitrogenated or non-nitrogenated bisphosphonates. Maintenance of graft structure and chondrocyte viability were assessed at 3 time points. A miniature swine trochlear defect model was used to evaluate the influence of bisphosphonate-augmented storage media on in vivo incorporation of fresh osteochondral tissue, which was quantified via μCT and decalcified histology. In the in vitro study, Safranin-O/Fast Green staining showed that both low- and high-dose nitrogenated-treated grafts retained chondrocyte viability and cartilage matrix for up to 43 days of storage. Allografts stored in nitrogenated-augmented storage media showed both μCT and histologic evidence of enhanced in vivo bony and cartilaginous incorporation in the miniature swine trochlear defect model. Several preclinical studies have shown the potential for enhanced storage of fresh osteochondral allografts via additions of relatively common drugs and biomolecules. This study showed that supplementing standard storage media with nitrogenated bisphosphonates may improve maintenance of chondrocyte viability and graft structure during cold storage as well as enhance in vivo osseous and cartilaginous incorporation of the graft. [ Orthopedics : 2018; 41(3):e376–e382.] |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|