A systems biology network analysis of nutri(epi)genomic changes in endothelial cells exposed to epicatechin metabolites

Autor: Katarzyna Szarc vel Szic, Laurent-Emmanuel Monfoulet, Chandra Sekhar Chirumamilla, Ken Declerck, Maija Lahtela-Kakkonen, Clarissa Gerhäuser, Sylvain Claude, Wim Vanden Berghe, Dragan Milenkovic, Annette M. van de Sandt, Christine Morand, Marc W. Merx, Simone Gorressen, Malte Kelm
Přispěvatelé: Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Department of Internal Medicine, Division of Cardiovascular Medicine, School of Medicine, University of California, PPES, Department of Biomedical Sciences, University of Antwerp (UA), L-GEST, Department of Biochemistry and Microbiology, UGent, Universiteit Gent = Ghent University [Belgium] (UGENT), Department of Medicine, Division of Cardiology, Pneumology and Angiology, University Hospital Düsseldorf, School of Pharmacy, University of Eastern Finland, Division of Epigenomics and Cancer Risk Factors, German Cancer Research Center - Deutsches Krebsforschungszentrum [Heidelberg] (DKFZ), COST (European Cooperation in Science and Technology) FA1403, European Project: 226588,EC:FP7:KBBE,FP7-KBBE-2008-2B,FLAVIOLA(2009), Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), b Department of Internal Medicine, Division of Cardiovascular Medicine, School of Medicine, Ghent University [Belgium] (UGENT), Unité Mixte de Recherches sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA), Milenkovic, Dragan, Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, University of California (UC), Universiteit Gent = Ghent University (UGENT), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Unité de Nutrition Humaine - Clermont Auvergne ( UNH ), Université Clermont Auvergne ( UCA ) -Institut national de la recherche agronomique [Auvergne/Rhône-Alpes] ( INRA Auvergne/Rhône-Alpes ), University of Antwerp ( UA ), Ghent University [Belgium] ( UGENT ), Unité Mixte de Recherches sur les Herbivores ( UMR 1213 Herbivores ), VetAgro Sup ( VAS ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique ( INRA ), German Cancer Research Center
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Male
Epigenomics
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
Metabolite
[SDV]Life Sciences [q-bio]
lcsh:Medicine
030204 cardiovascular system & hematology
Inbred C57BL
Cardiovascular
Catechin
Transcriptome
chemistry.chemical_compound
Mice
0302 clinical medicine
Nutrigenomics
Cell Movement
2.1 Biological and endogenous factors
Gene Regulatory Networks
Aetiology
lcsh:Science
nutrigénomique
Cytoskeleton
analyse in vivo
Multidisciplinary
Systems Biology
in vitro
Cell biology
Endothelial stem cell
Alimentation et Nutrition
endothelial cell
Metabolome
Bio-informatique
medicine.symptom
Engineering sciences. Technology
Biotechnology
Bioinformatics
Systems biology
1.1 Normal biological development and functioning
metabolite
épicatéchine
Inflammation
Leukocyte Rolling
Biotechnologies
nutragenomics
Article
03 medical and health sciences
Underpinning research
medicine
Cell Adhesion
Human Umbilical Vein Endothelial Cells
Food and Nutrition
Animals
Humans
Biology
[ SDV ] Life Sciences [q-bio]
Microcirculation
lcsh:R
Transendothelial and Transepithelial Migration
Epigenome
DNA Methylation
In vitro
Mice
Inbred C57BL
MicroRNAs
030104 developmental biology
chemistry
Gene Expression Regulation
lcsh:Q
Human medicine
[INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM]
cellule endotheliale
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Zdroj: Scientific Reports
Scientific Reports, Nature Publishing Group, 2018, 8 (1), ⟨10.1038/s41598-018-33959-x⟩
Scientific reports, vol 8, iss 1
Scientific reports
2nd COST POSITIVe Scientific Workshop on « Omics breakthroughs in the Health effects of plant food bioactives . 1 (8), 2017; 2. COST POSITIVe Scientific Workshop on « Omics breakthroughs in the Health effects of plant food bioactives, Thessalonique, GRC, 2017-09-20-2017-09-21
2. COST POSITIVe Scientific Workshop on « Omics breakthroughs in the Health effects of plant food bioactives
2. COST POSITIVe Scientific Workshop on « Omics breakthroughs in the Health effects of plant food bioactives, Sep 2017, Thessalonique, Greece. 2017
Scientific Reports 1 (8), . (2018)
Scientific Reports, Vol 8, Iss 1, Pp 1-17 (2018)
2. COST POSITIVe Scientific Workshop on « Omics breakthroughs in the Health effects of plant food bioactives, Sep 2017, Thessalonique, Greece. Scientific Reports, 8 (1), 2017, 2nd COST POSITIVe Scientific Workshop on « Omics breakthroughs in the Health effects of plant food bioactives. ⟨10.1038/s41598-018-33959-x⟩
Scientific Reports, 2018, 8 (1), ⟨10.1038/s41598-018-33959-x⟩
ISSN: 2045-2322
DOI: 10.1038/s41598-018-33959-x⟩
Popis: Presented at 2. COST POSITIVe Scientific Workshop on « Omics breakthroughs in the Health effects of plant food bioactives ; Thessalonique (Grèce) - (2017-09-20 - 2017-09-21) / Workshop (Prodinra 408917); International audience; Although vasculo-protective effects of flavan-3-ols are widely accepted today, their impact on endothelial cell function and underlying molecular mechanisms of action remain unexplored. The aim of this study was to characterize the potential endothelium-protective effects of epicatechin metabolites and to define underlying mechanisms of action by an integrated systems biology approach. Upon dietary epicatechin supplementation in a mouse model of inflammation, reduced leukocyte rolling over vascular endothelium was observed. Pathway enrichment analysis of transcriptome, epigenome and miRNome profiles of endothelial cells exposed to plasma concentrations of epicatechin metabolites suggested significant changes in interactions with immune cell as well as permeability regarding immune cell transmigration. In line with our in-silico network analysis, in-vitro experiments confirmed that epicatechin metabolites reduce monocyte adhesion to endothelial cells and their transendothelial migration. Altogether, our in-vivo and in-vitro results support the outcome of a systems biology based network analysis of epigenome, transcriptome and miRNome profiles, which suggests that epicatechin metabolites mediate their vasculoprotective effects through dynamic regulation of endothelial cell monocyte adhesion and permeability. This study illustrates complex and multimodal mechanisms of action by which epicatechin modulate endothelial cell integrity.
Databáze: OpenAIRE
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