In Vitro Metabolism of 17β-Estradiol by Human Liver Tissue
| Autor: | Mona Nilsen, R. Hobkirk, Joyce D. Mellor |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Adult
medicine.medical_specialty Uridine diphosphate glucuronic acid Estradiol Human liver medicine.drug_class In vitro metabolism Metabolite Phosphate buffered saline Glucuronates General Medicine Biology Glucuronic acid Culture Media chemistry.chemical_compound Endocrinology Liver chemistry Biochemistry Estrogen Internal medicine medicine Humans |
| Zdroj: | Canadian Journal of Biochemistry. 53:903-906 |
| ISSN: | 0008-4018 |
| DOI: | 10.1139/o75-123 |
| Popis: | 17β-[6,7-3H]Estradiol was incubated with adult human liver slices in Krebs–Ringer phosphate buffer containing glucose. Of the identified 3H recovered, 51–76% consisted of estrone-3-sulfate (E13S) and 17β-estradiol-3-sulfate (E23S). E13S was the main metabolite and was found in both tissue and medium. E23S was present only in the medium. Minor amounts of estrogen glucuronides were formed. When a human liver homogenate was incubated with [3H]E2 in a medium fortified with excess uridine diphosphate glucuronic acid only some 4% of conjugation with glucuronic acid was observed. It is suggested that human liver favors sulfurylation as the conjugating mechanism for E2 and E1. |
| Databáze: | OpenAIRE |
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