Near-infrared fluorescence imaging-guided focused ultrasound-mediated therapy against Rheumatoid Arthritis by MTX-ICG-loaded iRGD-modified echogenic liposomes

Autor: Hongmei Liu, Mei-Jun Zhou, Fei Yan, Haohan Wu, Hao Wu, Yanni He, Ran Xiong, Zhili Xu
Rok vydání: 2020
Předmět:
Male
Near-Infrared Fluorescence Imaging
Fluorescence-lifetime imaging microscopy
Swine
Angiogenesis
Ultrasonic Therapy
medicine.medical_treatment
Medicine (miscellaneous)
02 engineering and technology
01 natural sciences
Targeted therapy
Echogenic liposomes
Arthritis
Rheumatoid
Mice
chemistry.chemical_compound
Pharmacology
Toxicology and Pharmaceutics (miscellaneous)
Spectroscopy
Near-Infrared
Optical Imaging
021001 nanoscience & nanotechnology
iRGD
Ultrasonic Waves
Rheumatoid arthritis
0210 nano-technology
Oligopeptides
Research Paper
medicine.drug
Indocyanine Green
musculoskeletal diseases
Near-infrared fluorescence imaging
010402 general chemistry
Cell Line
Ultrasound-controlled drug release
Synovitis
Human Umbilical Vein Endothelial Cells
medicine
Animals
Humans
Fluorescent Dyes
business.industry
Endothelial Cells
Integrin alphaVbeta3
medicine.disease
0104 chemical sciences
Methotrexate
RAW 264.7 Cells
chemistry
Liposomes
Cancer research
business
Indocyanine green
Zdroj: Theranostics
ISSN: 1838-7640
DOI: 10.7150/thno.44865
Popis: Rheumatoid arthritis (RA), a common inflammatory disorder of the joints characterized by synovitis and pannus formation, often results in irreversible joint erosion and disability. Methotrexate (MTX) is the first-line drug against RA, but the therapeutic effects are sub-optimal due to its poor retention at the target sites and systemic side effects. Multifunctional nanoparticles are highly promising agents for minimally invasive, traceable and effective targeted therapy. Methods: This study developed iRGD peptide-functionalized echogenic liposomes (iELPs) which encapsulates MTX and indocyanine green (ICG) fluorescent probe through the thin film-hydration method. Results: The resulting iELPs showed high affinity for endothelial cells overexpressing αvβ3 integrin, favorable acoustic response and fluorescence tracking properties. Also, near-infrared (NIR) fluorescence imaging of iELPs and ultrasound-triggered drug release of MTX were proved in a mouse RA model, greatly improving the therapeutic efficacy and reducing MTX side effects. Histological assessment of the articular tissues further revealed significantly lower inflammatory cell infiltration and angiogenesis in the iELPs-treated and sonicated mice. Conclusion: Our study provides a promising nanoplatform for integrating ultrasound-controlled drug release and NIR fluorescence imaging for RA treatment.
Databáze: OpenAIRE
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