The plastoquinone pool of Nannochloropsis oceanica is not completely reduced during bright light pulses

Autor: Thor Bernt Melø, Gunvor Røkke, Martin F. Hohmann-Marriott
Rok vydání: 2017
Předmět:
Chlorophyll
Pigments
0106 biological sciences
0301 basic medicine
Luminescence
Chloroplasts
Light
Plastoquinone
lcsh:Medicine
Plant Science
01 natural sciences
chemistry.chemical_compound
Cell Signaling
lcsh:Science
Light Pulses
Multidisciplinary
biology
Physics
Electromagnetic Radiation
Plants
Fluorescence
Chemistry
Biochemistry
Physical Sciences
Cellular Structures and Organelles
Cellular Types
Oxidation-Reduction
Elementary Particles
Stramenopiles
Nannochloropsis
Research Article
Chemical Elements
Signal Transduction
Chlorophyll a
Algae
Plant Cell Biology
Materials Science
Photosynthesis
Fluorescence spectroscopy
03 medical and health sciences
Plant Cells
Particle Physics
Materials by Attribute
Photons
Organic Pigments
lcsh:R
Organisms
Biology and Life Sciences
Cell Biology
biology.organism_classification
Oxygen
Kinetics
030104 developmental biology
chemistry
Photoprotection
Biophysics
lcsh:Q
010606 plant biology & botany
Zdroj: PLoS ONE
PLoS ONE, Vol 12, Iss 4, p e0175184 (2017)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0175184
Popis: The lipid-producing model alga Nannochloropsis oceanica has a distinct photosynthetic machinery. This organism possesses chlorophyll a as its only chlorophyll species, and has a high ratio of PSI to PSII. This high ratio of PSI to PSII may affect the redox state of the plastoquinone pool during exposure to light, and consequently may play a role in activating photoprotection mechanisms. We utilized pulse-amplitude modulated fluorometry to investigate the redox state of the plastoquinone pool during and after bright light pulses. Our data indicate that even very intense (5910 μmol photons s-1m-2 of blue light having a wavelength of 440 nm) light pulses of 0.8 second duration are not sufficient to completely reduce the plastoquinone pool in Nannochloropsis. In order to achieve extensive reduction of the plastoquinone pool by bright light pulses, anaerobic conditions or an inhibitor of the photosynthetic electron transport chain has to be utilized. The implication of this finding for the application of the widely used saturating pulse method in algae is discussed. © 2017 Røkke et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Databáze: OpenAIRE
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