A novel amperometric enzyme inhibition biosensor based on xanthine oxidase immobilised onto glassy carbon electrodes for bisphenol A determination
| Autor: | Cherif Dridi, Mariana Emilia Ghica, Christopher M.A. Brett, Najib Ben Messaoud, Mounir Ben Ali |
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| Rok vydání: | 2018 |
| Předmět: |
Xanthine Oxidase
endocrine system Biosensing Techniques macromolecular substances 02 engineering and technology Glassy carbon 01 natural sciences Analytical Chemistry Electrolytes chemistry.chemical_compound Phenols Rivers Benzhydryl Compounds Enzyme Inhibitors Xanthine oxidase Electrodes Hypoxanthine Detection limit Minerals Chromatography 010401 analytical chemistry technology industry and agriculture Substrate (chemistry) Electrochemical Techniques Hydrogen-Ion Concentration Enzymes Immobilized 021001 nanoscience & nanotechnology Carbon Amperometry 0104 chemical sciences chemistry Glutaral Glass Glutaraldehyde 0210 nano-technology Biosensor Water Pollutants Chemical |
| Zdroj: | Talanta. 184:388-393 |
| ISSN: | 0039-9140 |
| Popis: | A novel and simple biosensor for the determination of bisphenol A (BPA) based on xanthine oxidase (XOD) enzymatic inhibition has been developed. The biosensor was prepared from xanthine oxidase immobilised by crosslinking with glutaraldehyde, with hypoxanthine as enzyme substrate, and was successfully applied to the determination of BPA using fixed potential amperometry. Biosensor performance was optimised with respect to the applied potential, influence of pH of the electrolyte solution, XOD loading and the substrate concentration. The enzyme inhibition mechanism was evaluated from Cornish-Bowden plus Dixon plots and was found to be reversible and competitive with an apparent inhibition constant of 8.15 nM. Under optimised conditions, the determination of BPA can be achieved in the linear range up to 41 nM with a detection limit of 1.0 nM, which is equal to the lowest reported in the literature, with very good repeatability and reproducibility. The selectivity of the biosensor was evaluated by performing an interference study and found to be excellent; and stability was investigated. It was successfully applied to the detection of BPA in mineral water and in river water. |
| Databáze: | OpenAIRE |
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