Mutagenicity of 5-formylcytosine, an oxidation product of 5-methylcytosine, in DNA in mammalian cells
| ISSN: | 0021-924X |
|---|---|
| Přístupová URL adresa: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::59f1ad99c529f3f0c4b0c21b7d4675e6 https://pubmed.ncbi.nlm.nih.gov/12359069 |
| Přírůstkové číslo: | edsair.doi.dedup.....59f1ad99c529f3f0c4b0c21b7d4675e6 |
| Autor: | Yoshihito Ueno, Hideyoshi Harashima, Hiroyuki Tsuchiya, Naoko Karino, Akira Matsuda, Hiroyuki Kamiya |
| Rok vydání: | 2002 |
| Předmět: |
DNA Replication
Genetic Vectors Biology medicine.disease_cause Transfection Biochemistry chemistry.chemical_compound Cytosine Shuttle vector Chlorocebus aethiops medicine Animals Molecular Biology Mutation Base Sequence DNA replication General Medicine Templates Genetic Molecular biology Restriction enzyme 5-Methylcytosine chemistry Oligodeoxyribonucleotides Coding strand COS Cells Mutagenesis Site-Directed Oxidation-Reduction DNA DNA Damage Mutagens Plasmids |
| Zdroj: | Journal of biochemistry. 132(4) |
| ISSN: | 0021-924X |
| Popis: | To examine the mutagenicity of 5-formylcytosine (5-fC), an oxidation product of 5-methylcytosine (5-mC), 5-fC was incorporated into predetermined sites of double-stranded shuttle vectors. The nucleotide sequences in which the modified base was incorporated were 5'-AFGCGT-3' and 5'-ACGFGT-3' (F represents 5-fC), the recognition site for the restriction enzyme MluI (5'-ACGCGT-3'). 5-fC was incorporated into the template strand of either the leading or lagging strand of DNA replication. The modified DNAs were transfected into simian COS-7 cells, and the DNAs replicated in the cells were recovered and analyzed after a second transfection into Escherichia coli. 5-fC weakly blocked DNA replication in mammalian cells. The 5-fC residues were mutagenic, with mutation frequencies in double-stranded vectors of 0.03-0.28%. The mutation spectrum of 5-fC was broad, and included targeted (5-fC-->G, 5-fC-->A, and 5-fC-->T) and untargeted mutations. These results suggest that the oxidation of 5-mC results in mutations at and around the modified sites. |
| Databáze: | OpenAIRE |
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