Distribution of water-soluble crystallins in microsectioned cataractous lenses from one hundred Egyptian patients
| Autor: | A.A. El-Layeh, M.H. Emarah, H. Rink, Johan Bours |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Adult
Male medicine.medical_specialty Aging genetic structures Population Normal aging Biology Cataract law.invention Cellular and Molecular Neuroscience Cataracts law Crystallin Ophthalmology Lens Crystalline medicine Humans education Aged Aged 80 and over education.field_of_study Chromatography Isoelectric focusing Water General Medicine Microtomy Middle Aged medicine.disease Crystallins eye diseases Sensory Systems Lens (optics) Molecular Weight Water soluble Solubility Normal lens Egypt Female sense organs Isoelectric Focusing |
| Zdroj: | Ophthalmic research. 27 |
| ISSN: | 0030-3747 |
| Popis: | Morphologically classified lenses from 100 cataract patients from the Mansoura Eye Hospital (Egypt) were microdissected and the crystallin composition and distribution were analyzed by thin-layer isoelectric focusing (IEF). The IEF profiles of cataractous lenses were compared with each other, with those of sclerotic lenses and with a normal lens profile. The alterations in the composition of high-molecular-weight (HMW)-, alpha-, beta H-, beta L-, beta s-, and gamma-crystallins along with normal aging, are superimposed by pronounced cataract-related changes which are different for the various types of cataracts. The general feature includes a continuous loss of gamma-, beta s- and beta L-crystallins of higher IEPs and an increase of HMW material. This is highly pronounced in the nucleus of nuclear cataractous lenses. In cortical cataractous lenses, changes start in the cortical layers. No differences could be observed between alterations in Caucasian lenses and this extended Egyptian lens population. |
| Databáze: | OpenAIRE |
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